Fibrochondrocyte Growth and Functionality on TiO2 Nanothin Films
AbstractDisorders affecting the temporomandibular joint (TMJ) are a long-standing health concern. TMJ disorders (TMJD) are often associated with an internal disc derangement accompanied by a suite of symptoms including joint noises, jaw dysfunction, and severe pain. The severity of patient symptoms and their reoccurrence can be alleviated to some extent with conservative therapy; however, refractory cases often require surgery that has shown only limited success. Bioengineered scaffolds with cell supportive surfaces an d nanoarchitectures that mimic TMJ tissue structure may offer an alternative treatment modality. In this study, titanium dioxide (TiO2) nanothin films, fabricated by layer-by-layer assembly, were examined as means for creating such a scaffold. The viability and growth of TMJ discal fibrochondrocytes (FCs) were assessed through MTT and DNA assays and total protein content over a 14-day experimental period. ELISA was also used to measure expression of types I and II collagen, decorin and aggrecan. Quantitative analyses demonstrated that FCs synthesized characteristic discal matrix proteins, with an increased production of type I collagen and decorin as opposed to collagen type II and aggrecan. A stimulatory effect on discal FC proliferation and extracellular matrix (ECM) expression with thicker nanofilms was also observed. The cumulative results suggest that TiO2 nanofilms may have potential as a TMJ scaffolding material. View Full-Text
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Ronald, S.; Mills, D.K. Fibrochondrocyte Growth and Functionality on TiO2 Nanothin Films. J. Funct. Biomater. 2016, 7, 15.
Ronald S, Mills DK. Fibrochondrocyte Growth and Functionality on TiO2 Nanothin Films. Journal of Functional Biomaterials. 2016; 7(2):15.Chicago/Turabian Style
Ronald, Sharon; Mills, David K. 2016. "Fibrochondrocyte Growth and Functionality on TiO2 Nanothin Films." J. Funct. Biomater. 7, no. 2: 15.
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