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J. Funct. Biomater. 2015, 6(2), 367-378; doi:10.3390/jfb6020367

Biomimetic Hybrid Nanofiber Sheets Composed of RGD Peptide-Decorated PLGA as Cell-Adhesive Substrates

1
Department of Optics and Mechatronics Engineering, BK21+ Nano-Integrated Cogno-Mechatronics Engineering, College of Nanoscience & Nanotechnology, Pusan National University, Busandaehak-ro 63beon-gil, Geumjeong-gu, Busan 609-735, Korea
2
Department of Nanoenergy Engineering, College of Nanoscience & Nanotechnology, Pusan National University, Busandaehak-ro 63beon-gil, Geumjeong-gu, Busan 609-735, Korea
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Academic Editor: Francesco Puoci
Received: 27 April 2015 / Revised: 21 May 2015 / Accepted: 28 May 2015 / Published: 29 May 2015
(This article belongs to the Special Issue Feature Papers)
View Full-Text   |   Download PDF [1399 KB, uploaded 29 May 2015]   |  

Abstract

In biomedical applications, there is a need for tissue engineering scaffolds to promote and control cellular behaviors, including adhesion, proliferation and differentiation. In particular, the initial adhesion of cells has a great influence on those cellular behaviors. In this study, we concentrate on developing cell-adhesive substrates applicable for tissue engineering scaffolds. The hybrid nanofiber sheets were prepared by electrospinning poly(lactic-co-glycolic acid) (PLGA) and M13 phage, which was genetically modified to enhance cell adhesion thru expressing RGD peptides on their surface. The RGD peptide is a specific motif of extracellular matrix (ECM) for integrin receptors of cells. RGD peptide-decorated PLGA (RGD-PLGA) nanofiber sheets were characterized by scanning electron microscopy, immunofluorescence staining, contact angle measurement and differential scanning calorimetry. In addition, the initial adhesion and proliferation of four different types of mammalian cells were determined in order to evaluate the potential of RGD-PLGA nanofiber sheets as cell-adhesive substrates. Our results showed that the hybrid nanofiber sheets have a three-dimensional porous structure comparable to the native ECM. Furthermore, the initial adhesion and proliferation of cells were significantly enhanced on RGD-PLGA sheets. These results suggest that biomimetic RGD-PLGA nanofiber sheets can be promising cell-adhesive substrates for application as tissue engineering scaffolds. View Full-Text
Keywords: tissue engineering scaffolds; cell-adhesive substrates; hybrid nanofiber sheets; electrospinning; poly(lactic-co-glycolic acid); RGD peptides tissue engineering scaffolds; cell-adhesive substrates; hybrid nanofiber sheets; electrospinning; poly(lactic-co-glycolic acid); RGD peptides
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Shin, Y.C.; Lee, J.H.; Kim, M.J.; Park, J.H.; Kim, S.E.; Kim, J.S.; Oh, J.-W.; Han, D.-W. Biomimetic Hybrid Nanofiber Sheets Composed of RGD Peptide-Decorated PLGA as Cell-Adhesive Substrates. J. Funct. Biomater. 2015, 6, 367-378.

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