Poly(2-hydroxyethyl methacrylate)-quercetin Conjugate as Biomaterial in Ophthalmology: An “ab initio” Study
Abstract
:1. Introduction
2. Experimental Section
2.1. Materials
2.2. Instruments
2.3. Synthesis of pHEMA-Q Conjugate
2.4. Ocular Tolerance Test (HET-CAM Test)
Effect | Score | Inference |
---|---|---|
No visible hemorrhage | 0 | Non irritant |
Just visible membrane discoloration | 1 | Mild irritant |
Structures are covered partially due to membrane discoloration or hemorrhage | 2 | Moderately irritant |
Structures are covered totally due to membrane discoloration or hemorrhage | 3 | Severe irritant |
2.5. Evaluation of Disposable Phenolic Groups by Folin-Ciocalteu Procedure
2.6. Hydrophilic Properties of Polymers
2.7. Determination of Scavenging Effect on the DPPH Radical
2.8. Determination of Total Antioxidant Activity
2.9. β-Carotene-Linoleic Acid Assay
2.10. Determination of Scavenging Properties on Peroxynitrite Anion
2.11. Scavenging Activity on Hydroxyl Radical
2.12. Anti-Inflammatory Effect
2.13. Statistical Analyses
3. Results and Discussion
3.1. Synthesis and Characterization of the pHEMA-Q Conjugate
3.2. Evaluation of the Antioxidant and Anti Inflammatory Properties
4. Conclusions
Acknowledgments
References and Notes
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Curcio, M.; Cirillo, G.; Parisi, O.I.; Iemma, F.; Spizzirri, U.G.; Altimari, I.; Picci, N.; Puoci, F. Poly(2-hydroxyethyl methacrylate)-quercetin Conjugate as Biomaterial in Ophthalmology: An “ab initio” Study. J. Funct. Biomater. 2011, 2, 1-17. https://doi.org/10.3390/jfb2010001
Curcio M, Cirillo G, Parisi OI, Iemma F, Spizzirri UG, Altimari I, Picci N, Puoci F. Poly(2-hydroxyethyl methacrylate)-quercetin Conjugate as Biomaterial in Ophthalmology: An “ab initio” Study. Journal of Functional Biomaterials. 2011; 2(1):1-17. https://doi.org/10.3390/jfb2010001
Chicago/Turabian StyleCurcio, Manuela, Giuseppe Cirillo, Ortensia Ilaria Parisi, Francesca Iemma, Umile Gianfranco Spizzirri, Ilaria Altimari, Nevio Picci, and Francesco Puoci. 2011. "Poly(2-hydroxyethyl methacrylate)-quercetin Conjugate as Biomaterial in Ophthalmology: An “ab initio” Study" Journal of Functional Biomaterials 2, no. 1: 1-17. https://doi.org/10.3390/jfb2010001