Next Article in Journal
The Tsunami Vulnerability Assessment of Urban Environments through Freely Available Datasets: The Case Study of Napoli City (Southern Italy)
Next Article in Special Issue
Oil Characterization and Distribution in Florida Estuary Sediments Following the Deepwater Horizon Spill
Previous Article in Journal
Comparison of the Performance of Two Advanced Spectral Methods for the Analysis of Times Series in Paleoceanography
Article Menu

Export Article

Open AccessArticle
J. Mar. Sci. Eng. 2015, 3(3), 968-980; doi:10.3390/jmse3030968

RNA-TGGE, a Tool for Assessing the Potential for Bioremediation in Impacted Marine Ecosystems

1
Centre for Environmental Sustainability and Remediation, School of Applied Sciences, RMIT University, PO Box 71, Bundoora 3083, Australia
2
School of Biological Sciences, Flinders University, GPO Box 2100, Adelaide 5001, Australia
*
Author to whom correspondence should be addressed.
Academic Editor: Merv Fingas
Received: 18 June 2015 / Accepted: 26 August 2015 / Published: 31 August 2015
(This article belongs to the Special Issue Marine Oil Spills)
View Full-Text   |   Download PDF [1454 KB, uploaded 31 August 2015]   |  

Abstract

Cultivation-independent genomic approaches have greatly advanced our understanding of the ecology and diversity of microbial communities involved in biodegradation processes. However, much still needs to be resolved in terms of the structure, composition and dynamics of the microbial community in impacted ecosystems. Here we report on the RNA activity of the microbial community during the bioremediation process using RNA Temperature Gradient Gel Electrophoresis (RNA-TGGE). Dendrograms constructed from similarity matching data produced from the TGGE profiles separated a community exhibiting high remediation potential. Overall, increased Shannon Weaver Diversity indices (1–2.4) were observed in the high potential remediation treatment samples. The functionality of the microbial community was compared, with the microbial community showing the greatest organisation also showing the highest levels of hydrocarbon degradation. Subsequent sequencing of excised bands from the microbial community identified the presence of Gammaproteobacteria together with a number of uncultured bacteria. The data shows that RNA TGGE represents a simple, reproducible and effective tool for use in the assessment of a commercial bioremediation event, in terms of monitoring either the natural or augmented hydrocarbon-degrading microbial community. View Full-Text
Keywords: bioremediation; microbial community dynamics; Pareto-Lorenz curve; RNA; Shannon Weaver diversity; TGGE bioremediation; microbial community dynamics; Pareto-Lorenz curve; RNA; Shannon Weaver diversity; TGGE
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Kadali, K.K.; Shahsavari, E.; Simons, K.L.; Sheppard, P.J.; Ball, A.S. RNA-TGGE, a Tool for Assessing the Potential for Bioremediation in Impacted Marine Ecosystems. J. Mar. Sci. Eng. 2015, 3, 968-980.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
J. Mar. Sci. Eng. EISSN 2077-1312 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top