In Vitro and In Vivo Effects of Gracilaria verrucosa Extracts on Osteoclast Differentiation
AbstractBone remodeling, a physiological process characterized by bone formation by osteoblasts and bone resorption by osteoclasts, is important for the maintenance of healthy bone in adult humans. Osteoclasts play a critical role in bone erosion and osteoporosis and are bone-specific multinucleated cells generated through differentiation of monocyte/macrophage lineage precursors. Receptor activator of NF-κB ligand (RANKL) has been reported to induce osteoclast differentiation. In this study, we explored whether Gracilaria verrucosa extracts (GE) could affect RANKL-mediated osteoclast differentiation. GE significantly inhibited RANKL-activated osteoclast differentiation by inhibiting protein expression of c-fos and nuclear factor of activated T-cells, cytoplasmic 1 (NFATc1), vital factors in RANKL-mediated osteoclastogenesis. In addition, GE attenuated ovariectomy-induced bone loss in mice. In summary, GE can prevent osteoclastogenesis and hormone-related bone loss via blockage of c-fos-NFATc1 signaling. Our results suggest that GE may have therapeutic potential in the treatment of postmenopausal osteoporosis. View Full-Text
- Supplementary File 1:
DOC-Document (DOC, 191 KB)
Share & Cite This Article
Kim, K.-J.; Lee, Y.-J.; Hwang, Y.-H.; Kang, K.-Y.; Yee, S.-T.; Son, Y.-J. In Vitro and In Vivo Effects of Gracilaria verrucosa Extracts on Osteoclast Differentiation. J. Clin. Med. 2017, 6, 32.
Kim K-J, Lee Y-J, Hwang Y-H, Kang K-Y, Yee S-T, Son Y-J. In Vitro and In Vivo Effects of Gracilaria verrucosa Extracts on Osteoclast Differentiation. Journal of Clinical Medicine. 2017; 6(3):32.Chicago/Turabian Style
Kim, Kwang-Jin; Lee, Yong-Jin; Hwang, Yun-Ho; Kang, Kyung-Yun; Yee, Sung-Tae; Son, Young-Jin. 2017. "In Vitro and In Vivo Effects of Gracilaria verrucosa Extracts on Osteoclast Differentiation." J. Clin. Med. 6, no. 3: 32.
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.