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Correction published on 20 November 2017, see J. Clin. Med. 2017, 6(11), 106.

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J. Clin. Med. 2017, 6(10), 91; doi:10.3390/jcm6100091

Comparison of Minimal Residual Disease Detection by Multiparameter Flow Cytometry, ASO-qPCR, Droplet Digital PCR, and Deep Sequencing in Patients with Multiple Myeloma Who Underwent Autologous Stem Cell Transplantation

Hematology/Respiratory Medicine, Faculty of Medicine, Institute of Medical, Pharmaceutical and Health Sciences, Kanazawa University, 13-1 Takaramachi, Kanazawa, Ishikawa 920-8641, Japan
Academic Editor: Marco Ladetto
Received: 30 April 2017 / Revised: 17 September 2017 / Accepted: 20 September 2017 / Published: 25 September 2017
(This article belongs to the Special Issue Role of Minimal Residual Disease Assessment in Hematological Cancers)
View Full-Text   |   Download PDF [1844 KB, uploaded 27 September 2017]   |  

Abstract

Multiple myeloma (MM) is a hematological malignancy with a poor prognosis, characterized by clonal proliferation of plasma cells in the bone marrow (BM). Relapse due to undetected minimal residual disease (MRD) is the leading cause of death among patients with MM. This review summarizes the methods and prognostic value of MRD assessment in BM and autografts from MM patients who underwent autologous stem cell transplantation (ASCT) by multiparameter flow cytometry (MFC), allele-specific oligonucleotide real-time quantitative PCR (ASO-qPCR), droplet digital PCR (ddPCR), and next-generation sequencing (NGS)-based detection methods. MRD assessment using NGS-based approaches has clear prognostic value and better sensitivity compared to traditional methods. View Full-Text
Keywords: multiple myeloma; minimal residual disease; allele-specific oligonucleotide-PCR; droplet digital PCR; next-generation sequencing multiple myeloma; minimal residual disease; allele-specific oligonucleotide-PCR; droplet digital PCR; next-generation sequencing
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Takamatsu, H. Comparison of Minimal Residual Disease Detection by Multiparameter Flow Cytometry, ASO-qPCR, Droplet Digital PCR, and Deep Sequencing in Patients with Multiple Myeloma Who Underwent Autologous Stem Cell Transplantation. J. Clin. Med. 2017, 6, 91.

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