Next Article in Journal
Short Review on Predicting Fouling in RO Desalination
Previous Article in Journal
Characterizing the Performance of Gas-Permeable Membranes as an Ammonia Recovery Strategy from Anaerobically Digested Dairy Manure
Article Menu

Export Article

Open AccessArticle
Membranes 2017, 7(4), 60; doi:10.3390/membranes7040060

Revealing the Effects of Nanoscale Membrane Curvature on Lipid Mobility

Department of Physics and Astronomy, Wayne State University, Detroit, MI 48201, USA
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Received: 12 September 2017 / Revised: 29 September 2017 / Accepted: 12 October 2017 / Published: 18 October 2017
(This article belongs to the Section Membranes Transport Phenomena)
View Full-Text   |   Download PDF [2766 KB, uploaded 19 October 2017]   |  

Abstract

Recent advances in nanoengineering and super-resolution microscopy have enabled new capabilities for creating and observing membrane curvature. However, the effects of curvature on single-lipid diffusion have yet to be revealed. The simulations presented here describe the capabilities of varying experimental methods for revealing the effects of nanoscale curvature on single-molecule mobility. Traditionally, lipid mobility is revealed through fluorescence recovery after photobleaching (FRAP), fluorescence correlation spectroscopy (FCS), and single particle tracking (SPT). However, these techniques vary greatly in their ability to detect the effects of nanoscale curvature on lipid behavior. Traditionally, FRAP and FCS depend on diffraction-limited illumination and detection. A simulation of FRAP shows minimal effects on lipids diffusion due to a 50 nm radius membrane bud. Throughout the stages of the budding process, FRAP detected minimal changes in lipid recovery time due to the curvature versus flat membrane. Simulated FCS demonstrated small effects due to a 50 nm radius membrane bud that was more apparent with curvature-dependent lipid mobility changes. However, SPT achieves a sub-diffraction-limited resolution of membrane budding and lipid mobility through the identification of the single-lipid positions with ≤15 nm spatial and ≤20 ms temporal resolution. By mapping the single-lipid step lengths to locations on the membrane, the effects of membrane topography and curvature could be correlated to the effective membrane viscosity. Single-fluorophore localization techniques, such SPT, can detect membrane curvature and its effects on lipid behavior. These simulations and discussion provide a guideline for optimizing the experimental procedures in revealing the effects of curvature on lipid mobility and effective local membrane viscosity. View Full-Text
Keywords: fluorescence recovery after photobleaching; fluorescence correlation spectroscopy; single-particle tracking; supported lipid bilayers; membrane curvature; diffusion fluorescence recovery after photobleaching; fluorescence correlation spectroscopy; single-particle tracking; supported lipid bilayers; membrane curvature; diffusion
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Supplementary material

Share & Cite This Article

MDPI and ACS Style

Kabbani, A.M.; Woodward, X.; Kelly, C.V. Revealing the Effects of Nanoscale Membrane Curvature on Lipid Mobility. Membranes 2017, 7, 60.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Membranes EISSN 2077-0375 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top