Descriptive Analysis of LAP1 Distribution and That of Associated Proteins throughout Spermatogenesis
AbstractSpermatogenesis comprises highly complex differentiation processes. Nuclear envelope (NE) proteins have been associated with these processes, including lamins, lamina-associated polypeptide (LAP) 2 and the lamin B-receptor. LAP1 is an important NE protein whose function has not been fully elucidated, but several binding partners allow predicting putative LAP1 functions. To date, LAP1 had not been associated with spermatogenesis. In this study, LAP1 expression and cellular/subcellular localization during spermatogenesis in human and mouse testes is established for the first time. The fact that LAP1 is expressed during nuclear elongation in spermiogenesis and is located at the spermatids’ centriolar pole is singularly important. LAP1 binds to members of the protein phosphatase 1 (PP1) family. Similar localization of LAP1 and PP1γ2, a testis-specific PP1 isoform, suggests a shared function for both proteins during spermiogenesis. Furthermore, this study suggests an involvement of LAP1 in manchette development and chromatin regulation possibly via interaction with acetylated α-tubulin and lamins, respectively. Taken together, the present results indicate that, by moving to the posterior pole in spermatids, LAP1 can contribute to the achievement of non-random, sperm-specific chromatin distribution, as well as modulate cellular remodeling during spermiogenesis. In addition, LAP1 seems to be associated with dynamic microtubule changes related to manchette formation and flagella development. View Full-Text
Share & Cite This Article
Serrano, J.B.; Martins, F.; Sousa, J.C.; Pereira, C.D.; van Pelt, A.M.M.; Rebelo, S.; da Cruz e Silva, O.A.B. Descriptive Analysis of LAP1 Distribution and That of Associated Proteins throughout Spermatogenesis. Membranes 2017, 7, 22.
Serrano JB, Martins F, Sousa JC, Pereira CD, van Pelt AMM, Rebelo S, da Cruz e Silva OAB. Descriptive Analysis of LAP1 Distribution and That of Associated Proteins throughout Spermatogenesis. Membranes. 2017; 7(2):22.Chicago/Turabian Style
Serrano, Joana B.; Martins, Filipa; Sousa, João C.; Pereira, Cátia D.; van Pelt, Ans M.M.; Rebelo, Sandra; da Cruz e Silva, Odete A.B. 2017. "Descriptive Analysis of LAP1 Distribution and That of Associated Proteins throughout Spermatogenesis." Membranes 7, no. 2: 22.
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.