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Membranes 2017, 7(2), 22; doi:10.3390/membranes7020022

Descriptive Analysis of LAP1 Distribution and That of Associated Proteins throughout Spermatogenesis

Neuroscience and Signaling Laboratory, Department of Medical Sciences, Institute for Biomedicine—iBiMED, University of Aveiro, 3810-193 Aveiro, Portugal
Life and Health Sciences Research Institute (ICVS), School of Health Sciences, University of Minho, 4710-057 Braga, Portugal
ICVS/3B’s—PT Government Associate Laboratory, University of Minho, 4710-057 Braga/Guimarães, Portugal
Center for Reproductive Medicine, Academic Medical Center, University of Amsterdam, 1105 AZ Amsterdam, The Netherlands
These authors contributed equally to this work.
Author to whom correspondence should be addressed.
Academic Editor: Terry Hébert
Received: 17 February 2017 / Revised: 12 March 2017 / Accepted: 2 April 2017 / Published: 7 April 2017
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Spermatogenesis comprises highly complex differentiation processes. Nuclear envelope (NE) proteins have been associated with these processes, including lamins, lamina-associated polypeptide (LAP) 2 and the lamin B-receptor. LAP1 is an important NE protein whose function has not been fully elucidated, but several binding partners allow predicting putative LAP1 functions. To date, LAP1 had not been associated with spermatogenesis. In this study, LAP1 expression and cellular/subcellular localization during spermatogenesis in human and mouse testes is established for the first time. The fact that LAP1 is expressed during nuclear elongation in spermiogenesis and is located at the spermatids’ centriolar pole is singularly important. LAP1 binds to members of the protein phosphatase 1 (PP1) family. Similar localization of LAP1 and PP1γ2, a testis-specific PP1 isoform, suggests a shared function for both proteins during spermiogenesis. Furthermore, this study suggests an involvement of LAP1 in manchette development and chromatin regulation possibly via interaction with acetylated α-tubulin and lamins, respectively. Taken together, the present results indicate that, by moving to the posterior pole in spermatids, LAP1 can contribute to the achievement of non-random, sperm-specific chromatin distribution, as well as modulate cellular remodeling during spermiogenesis. In addition, LAP1 seems to be associated with dynamic microtubule changes related to manchette formation and flagella development. View Full-Text
Keywords: nuclear envelope; lamina-associated polypeptide 1; lamins; protein phosphatase 1; manchette; spermatids nuclear envelope; lamina-associated polypeptide 1; lamins; protein phosphatase 1; manchette; spermatids

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Serrano, J.B.; Martins, F.; Sousa, J.C.; Pereira, C.D.; van Pelt, A.M.M.; Rebelo, S.; da Cruz e Silva, O.A.B. Descriptive Analysis of LAP1 Distribution and That of Associated Proteins throughout Spermatogenesis. Membranes 2017, 7, 22.

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