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Membranes 2016, 6(3), 36; doi:10.3390/membranes6030036

Biochip for the Detection of Bacillus anthracis Lethal Factor and Therapeutic Agents against Anthrax Toxins

1
Physical Measurement Laboratory, National Institute of Standards and Technology, Gaithersburg, MD 20899-8120, USA
2
NIST Center for Neutron Research, National Institute of Standards and Technology, Gaithersburg, MD 20899-8120, USA
3
Institute for Bioscience and Biotechnology Research, University of Maryland, Rockville, MD 20899, USA
4
US Army Medical Research Institute of Infectious Diseases, Fort Detrick, Frederick, MD 21702-5011, USA
*
Author to whom correspondence should be addressed.
Academic Editor: Michiaki Matsumoto
Received: 12 May 2016 / Revised: 13 June 2016 / Accepted: 14 June 2016 / Published: 24 June 2016
(This article belongs to the Special Issue Supported Lipid Membranes)
View Full-Text   |   Download PDF [3183 KB, uploaded 24 June 2016]   |  

Abstract

Tethered lipid bilayer membranes (tBLMs) have been used in many applications, including biosensing and membrane protein structure studies. This report describes a biosensor for anthrax toxins that was fabricated through the self-assembly of a tBLM with B. anthracis protective antigen ion channels that are both the recognition element and electrochemical transducer. We characterize the sensor and its properties with electrochemical impedance spectroscopy and surface plasmon resonance. The sensor shows a sensitivity similar to ELISA and can also be used to rapidly screen for molecules that bind to the toxins and potentially inhibit their lethal effects. View Full-Text
Keywords: anthrax; protective antigen; lethal factor; edema factor; therapeutic agents; screening; tethered bilayer membrane; biochip anthrax; protective antigen; lethal factor; edema factor; therapeutic agents; screening; tethered bilayer membrane; biochip
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Silin, V.; Kasianowicz, J.J.; Michelman-Ribeiro, A.; Panchal, R.G.; Bavari, S.; Robertson, J.W.F. Biochip for the Detection of Bacillus anthracis Lethal Factor and Therapeutic Agents against Anthrax Toxins. Membranes 2016, 6, 36.

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