Next Article in Journal
Recovery of Flavonoids from Orange Press Liquor by an Integrated Membrane Process
Previous Article in Journal
Auxiliary Subunits: Shepherding AMPA Receptors to the Plasma Membrane
Article Menu

Export Article

Open AccessArticle
Membranes 2014, 4(3), 491-508; doi:10.3390/membranes4030491

Isolation and Characterization of Glycophorin from Carp Red Blood Cell Membranes

1
Laboratory of Quality in Marine Products, Graduate School of Bioresources, Mie University, 1577 Kurima Machiya-cho, Tsu, Mie 514-8507, Japan
2
Toray Research Center, Inc., 1111 Tebiro, Kamakura, Kanagawa 248-8555, Japan
*
Author to whom correspondence should be addressed.
Received: 26 February 2014 / Revised: 22 July 2014 / Accepted: 25 July 2014 / Published: 8 August 2014
View Full-Text   |   Download PDF [6149 KB, uploaded 8 August 2014]   |  

Abstract

We isolated a high-purity carp glycophorin from carp erythrocyte membranes following extraction using the lithium diiodosalicylate (LIS)-phenol method and streptomycin treatment. The main carp glycophorin was observed to locate at the position of the carp and human band-3 proteins on an SDS-polyacrylamide gel. Only the N-glycolylneuraminic acid (NeuGc) form of sialic acid was detected in the carp glycophorin. The oligosaccharide fraction was separated into two components (P-1 and P-2) using a Glyco-Pak DEAE column. We observed bacteriostatic activity against five strains of bacteria, including two known fish pathogens. Fractions from the carp erythrocyte membrane, the glycophorin oligosaccharide and the P-1 also exhibited bacteriostatic activity; whereas the glycolipid fraction and the glycophorin fraction without sialic acid did not show the activity. The carp glycophorin molecules attach to the flagellum of V. anguillarum or the cell surface of M. luteus and inhibited bacterial growth. View Full-Text
Keywords: fish; red blood cell membranes; erythrocyte; glycophorin; oligosaccharide; sialic acid; N-glycolylneuraminic acid; bacteriostatic activity fish; red blood cell membranes; erythrocyte; glycophorin; oligosaccharide; sialic acid; N-glycolylneuraminic acid; bacteriostatic activity
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Aoki, T.; Chimura, K.; Nakao, N.; Mizuno, Y. Isolation and Characterization of Glycophorin from Carp Red Blood Cell Membranes. Membranes 2014, 4, 491-508.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Membranes EISSN 2077-0375 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top