A Novel Proline-Rich Cathelicidin from the Alpaca Vicugna pacos with Potency to Combat Antibiotic-Resistant Bacteria: Mechanism of Action and the Functional Role of the C-Terminal Region
Abstract
:1. Introduction
2. Materials and Methods
2.1. Identification of CATHL Genes in Camelidae WGS Database
2.2. Expression and Purification of the Antimicrobial Peptides
2.3. Bacterial Strains
2.4. Antimicrobial Assay
2.5. In Vitro Transcription/Translation Inhibition Assay
2.6. Assessment of Bacterial Membrane Permeabilization
2.7. Resistance Induction Experiments
2.8. Whole-Genome Sequencing
2.9. Molecular Cloning Procedures
2.10. Hemolysis and Cytotoxicity Assay
2.11. Cytokine Response to Cathelicidins on Human Cells In Vitro
3. Results and Discussion
3.1. Identification of Novel Proline-Rich Cathelicidins in Camelidae Species
3.2. N-Terminal Fragments of Proline-Rich Cathelicidin VicBac Inhibit Protein Biosynthesis in Bacteria
3.3. The Biological Activity of Cathelicidin VicBac and Its Truncated Analog
3.4. The Presence of the C-Terminal Hydrophobic Motif Prevents Bacterial Resistance to VicBac
3.5. Analysis of the Resistance Mechanisms to VicBac[1–22]
3.6. The Presence of the C-Terminal Part Does Not Provide Specific Immune-Modulatory Effects of the Cathelicidin VicBac but Enhances Them
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Panteleev, P.V.; Safronova, V.N.; Kruglikov, R.N.; Bolosov, I.A.; Bogdanov, I.V.; Ovchinnikova, T.V. A Novel Proline-Rich Cathelicidin from the Alpaca Vicugna pacos with Potency to Combat Antibiotic-Resistant Bacteria: Mechanism of Action and the Functional Role of the C-Terminal Region. Membranes 2022, 12, 515. https://doi.org/10.3390/membranes12050515
Panteleev PV, Safronova VN, Kruglikov RN, Bolosov IA, Bogdanov IV, Ovchinnikova TV. A Novel Proline-Rich Cathelicidin from the Alpaca Vicugna pacos with Potency to Combat Antibiotic-Resistant Bacteria: Mechanism of Action and the Functional Role of the C-Terminal Region. Membranes. 2022; 12(5):515. https://doi.org/10.3390/membranes12050515
Chicago/Turabian StylePanteleev, Pavel V., Victoria N. Safronova, Roman N. Kruglikov, Ilia A. Bolosov, Ivan V. Bogdanov, and Tatiana V. Ovchinnikova. 2022. "A Novel Proline-Rich Cathelicidin from the Alpaca Vicugna pacos with Potency to Combat Antibiotic-Resistant Bacteria: Mechanism of Action and the Functional Role of the C-Terminal Region" Membranes 12, no. 5: 515. https://doi.org/10.3390/membranes12050515