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Vaccines 2014, 2(4), 772-784; doi:10.3390/vaccines2040772

A Recombinant Raccoon Poxvirus Vaccine Expressing both Yersinia pestis F1 and Truncated V Antigens Protects Animals against Lethal Plague

1
National Wildlife Health Center, U.S. Geological Survey, 6006 Schroeder Rd., Madison, WI 53711, USA
2
Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin, Madison, WI 53706, USA
*
Author to whom correspondence should be addressed.
Received: 28 May 2014 / Revised: 6 September 2014 / Accepted: 28 September 2014 / Published: 27 October 2014
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Abstract

In previous studies, we demonstrated in mice and prairie dogs that simultaneous administration of two recombinant raccoon poxviruses (rRCN) expressing Yersinia pestis antigens (F1 and V307—a truncated version of the V protein) provided superior protection against plague challenge compared to individual single antigen constructs. To reduce costs of vaccine production and facilitate implementation of a sylvatic plague vaccine (SPV) control program for prairie dogs, a dual antigen construct is more desirable. Here we report the construction and characterization of a novel RCN-vectored vaccine that simultaneously expresses both F1 and V307 antigens. This dual antigen vaccine provided similar levels of protection against plague in both mice and prairie dogs as compared to simultaneous administration of the two single antigen constructs and was also shown to protect mice against an F1 negative strain of Y. pestis. The equivalent safety, immunogenicity and efficacy profile of the dual RCN-F1/V307 construct warrants further evaluation in field efficacy studies in sylvatic plague endemic areas. View Full-Text
Keywords: raccoon poxvirus; Yersinia pestis; plague; vaccine; prairie dogs raccoon poxvirus; Yersinia pestis; plague; vaccine; prairie dogs
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Rocke, T.E.; Kingstad-Bakke, B.; Berlier, W.; Osorio, J.E. A Recombinant Raccoon Poxvirus Vaccine Expressing both Yersinia pestis F1 and Truncated V Antigens Protects Animals against Lethal Plague. Vaccines 2014, 2, 772-784.

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