Next Article in Journal
Green Tea and Other Tea Polyphenols: Effects on Sebum Production and Acne Vulgaris
Next Article in Special Issue
Think Yellow and Keep Green—Role of Sulfanes from Garlic in Agriculture
Previous Article in Journal
Role of Redox Signaling and Inflammation in Skeletal Muscle Adaptations to Training
Previous Article in Special Issue
The Effects of Different Garlic-Derived Allyl Sulfides on Anaerobic Sulfur Metabolism in the Mouse Kidney
Article Menu

Export Article

Open AccessArticle
Antioxidants 2017, 6(1), 1; doi:10.3390/antiox6010001

The Effects of Allicin, a Reactive Sulfur Species from Garlic, on a Selection of Mammalian Cell Lines

1
Department of Plant Physiology, RWTH Aachen University, Worringer Weg 1, Aachen 52074, Germany
2
Laboratoire d’Immunologie biologique, Hôpital Cochin, Paris 75679, France
*
Author to whom correspondence should be addressed.
Academic Editors: Claus Jacob and Gregory Ian Giles
Received: 6 September 2016 / Revised: 29 November 2016 / Accepted: 19 December 2016 / Published: 26 December 2016
View Full-Text   |   Download PDF [2163 KB, uploaded 26 December 2016]   |  

Abstract

Garlic (Allium sativum L.) has been used as a spice and medicinal plant since ancient times. Garlic produces the thiol-reactive defence substance, allicin, upon wounding. The effects of allicin on human lung epithelium carcinoma (A549), mouse fibroblast (3T3), human umbilical vein endothelial cell (HUVEC), human colon carcinoma (HT29) and human breast cancer (MCF7) cell lines were tested. To estimate toxic effects of allicin, we used a standard MTT-test (methylthiazoltetrazolium) for cell viability and 3H-thymidine incorporation for cell proliferation. The glutathione pool was measured using monobromobimane and the formation of reactive species was identified using 2′,7′-dichlorofluoresceine-diacetate. The YO-PRO-1 iodide staining procedure was used to estimate apoptosis. Allicin reduced cell viability and cell proliferation in a concentration dependent manner. In the bimane test, it was observed that cells treated with allicin showed reduced fluorescence, suggesting glutathione oxidation. The cell lines tested differed in sensitivity to allicin in regard to viability, cell proliferation and glutathione oxidation. The 3T3 and MCF-7 cells showed a higher proportion of apoptosis compared to the other cell types. These data show that mammalian cell lines differ in their sensitivity and responses to allicin. View Full-Text
Keywords: garlic; Allium sativum; allicin; tumour cell lines; glutathione; redox garlic; Allium sativum; allicin; tumour cell lines; glutathione; redox
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Gruhlke, M.C.H.; Nicco, C.; Batteux, F.; Slusarenko, A.J. The Effects of Allicin, a Reactive Sulfur Species from Garlic, on a Selection of Mammalian Cell Lines. Antioxidants 2017, 6, 1.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Antioxidants EISSN 2076-3921 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top