Next Article in Journal
Flavonoids Affect Host-Microbiota Crosstalk through TLR Modulation
Next Article in Special Issue
Role of Oxidative Stress in HIV-1-Associated Neurocognitive Disorder and Protection by Gene Delivery of Antioxidant Enzymes
Previous Article in Journal
Syzyguim guineense Extracts Show Antioxidant Activities and Beneficial Activities on Oxidative Stress Induced by Ferric Chloride in the Liver Homogenate
Previous Article in Special Issue
Friedreich’s Ataxia: A Neuronal Point of View on the Oxidative Stress Hypothesis
Article Menu

Export Article

Open AccessReview
Antioxidants 2014, 3(4), 636-648; doi:10.3390/antiox3040636

Assessing Antioxidant Capacity in Brain Tissue: Methodologies and Limitations in Neuroprotective Strategies

1
Bioscience Technology, Holland College, 140 Weymouth Street, Charlottetown, PE, C1A 4Z1, Canada
2
School of Pharmacy, Memorial University of Newfoundland, 300 Prince Philip Drive, St. John's, NL, A1B 3V6, Canada
*
Author to whom correspondence should be addressed.
Received: 17 July 2014 / Revised: 24 July 2014 / Accepted: 2 September 2014 / Published: 13 October 2014
(This article belongs to the Special Issue Oxidative Stress and Neurodegenerative Diseases)
View Full-Text   |   Download PDF [422 KB, uploaded 13 October 2014]   |  

Abstract

The number of putative neuroprotective compounds with antioxidant activity described in the literature continues to grow. Although these compounds are validated using a variety of in vivo and in vitro techniques, they are often evaluated initially using in vitro cell culture techniques in order to establish toxicity and effective concentrations. Both in vivo and in vitro methodologies have their respective advantages and disadvantages, including, but not limited to, cost, time, use of resources and technical limitations. This review expands on the inherent benefits and drawbacks of in vitro and in vivo methods for assessing neuroprotection, especially in light of proper evaluation of compound efficacy and neural bioavailability. For example, in vivo studies can better evaluate the effects of protective compounds and/or its metabolites on various tissues, including the brain, in the whole animal, whereas in vitro studies can better discern the cellular and/or mechanistic effects of compounds. In particular, we aim to address the question of appropriate and accurate extrapolation of findings from in vitro experiment-where compounds are often directly applied to cellular extracts, potentially at higher concentrations than would ever cross the blood-brain barrier—to the more complex scenario of neuroprotection due to pharmacodynamics in vivo. View Full-Text
Keywords: antioxidant; bioavailability; cell culture; neuroprotection antioxidant; bioavailability; cell culture; neuroprotection
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Slemmer, J.E.; Weber, J.T. Assessing Antioxidant Capacity in Brain Tissue: Methodologies and Limitations in Neuroprotective Strategies. Antioxidants 2014, 3, 636-648.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Antioxidants EISSN 2076-3921 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top