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From the third issue of 2017, Microarrays has changed its name to High-Throughput.

Open AccessArticle
Microarrays 2016, 5(3), 22; doi:10.3390/microarrays5030022

Serology in the Digital Age: Using Long Synthetic Peptides Created from Nucleic Acid Sequences as Antigens in Microarrays

1
Section of Clinical Virology, Department of Medical Sciences, Uppsala University, Uppsala 751 85, Sweden
2
Laboratory of Clinical Microbiology, Uppsala University Hospital, Uppsala 751 85, Sweden
3
August Kirchenstein Institute of Microbiology and Virology, Riga Stradins University, Riga LV-1067, Latvia
4
Zoonosis Science Center, Department of Medical Biochemistry and Microbiology, Uppsala University, Uppsala 75123, Sweden
5
Deutsches Krebsforschungszentrum, Heidelberg 69121, Germany
6
Laboratory of Clinical Microbiology, Linköping University Hospital, Linköping 58185, Sweden
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Academic Editors: Massimo Negrini and Quan-Zhen Li
Received: 25 May 2016 / Revised: 23 July 2016 / Accepted: 5 August 2016 / Published: 10 August 2016
(This article belongs to the Special Issue High-Throughput Microarray for Protein Biomarker Discovery)
View Full-Text   |   Download PDF [1921 KB, uploaded 10 August 2016]   |  

Abstract

Background: Antibodies to microbes, or to autoantigens, are important markers of disease. Antibody detection (serology) can reveal both past and recent infections. There is a great need for development of rational ways of detecting and quantifying antibodies, both for humans and animals. Traditionally, serology using synthetic antigens covers linear epitopes using up to 30 amino acid peptides. Methods: We here report that peptides of 100 amino acids or longer (“megapeptides”), designed and synthesized for optimal serological performance, can successfully be used as detection antigens in a suspension multiplex immunoassay (SMIA). Megapeptides can quickly be created just from pathogen sequences. A combination of rational sequencing and bioinformatic routines for definition of diagnostically-relevant antigens can, thus, rapidly yield efficient serological diagnostic tools for an emerging infectious pathogen. Results: We designed megapeptides using bioinformatics and viral genome sequences. These long peptides were tested as antigens for the presence of antibodies in human serum to the filo-, herpes-, and polyoma virus families in a multiplex microarray system. All of these virus families contain recently discovered or emerging infectious viruses. Conclusion: Long synthetic peptides can be useful as serological diagnostic antigens, serving as biomarkers, in suspension microarrays. View Full-Text
Keywords: megapeptide; suspension microarray; immunoassay; suspension multiplex immunoassay (SMIA); emerging virus infections megapeptide; suspension microarray; immunoassay; suspension multiplex immunoassay (SMIA); emerging virus infections
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Rizwan, M.; Rönnberg, B.; Cistjakovs, M.; Lundkvist, Å.; Pipkorn, R.; Blomberg, J. Serology in the Digital Age: Using Long Synthetic Peptides Created from Nucleic Acid Sequences as Antigens in Microarrays. Microarrays 2016, 5, 22.

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