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From the third issue of 2017, Microarrays has changed its name to High-Throughput.

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Microarrays 2016, 5(2), 11; doi:10.3390/microarrays5020011

Living Cell Microarrays: An Overview of Concepts

Institute of Technical Chemistry, Leibniz University of Hannover, Callinstr. 5, Hannover 30167, Germany
These authors contributed equally to this work.
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Author to whom correspondence should be addressed.
Academic Editor: Holger Erfle
Received: 30 March 2016 / Revised: 9 May 2016 / Accepted: 11 May 2016 / Published: 26 May 2016
(This article belongs to the Special Issue Cell-Based Microarrays)
View Full-Text   |   Download PDF [1297 KB, uploaded 26 May 2016]   |  

Abstract

Living cell microarrays are a highly efficient cellular screening system. Due to the low number of cells required per spot, cell microarrays enable the use of primary and stem cells and provide resolution close to the single-cell level. Apart from a variety of conventional static designs, microfluidic microarray systems have also been established. An alternative format is a microarray consisting of three-dimensional cell constructs ranging from cell spheroids to cells encapsulated in hydrogel. These systems provide an in vivo-like microenvironment and are preferably used for the investigation of cellular physiology, cytotoxicity, and drug screening. Thus, many different high-tech microarray platforms are currently available. Disadvantages of many systems include their high cost, the requirement of specialized equipment for their manufacture, and the poor comparability of results between different platforms. In this article, we provide an overview of static, microfluidic, and 3D cell microarrays. In addition, we describe a simple method for the printing of living cell microarrays on modified microscope glass slides using standard DNA microarray equipment available in most laboratories. Applications in research and diagnostics are discussed, e.g., the selective and sensitive detection of biomarkers. Finally, we highlight current limitations and the future prospects of living cell microarrays. View Full-Text
Keywords: living cell microarrays (LCMAs); transfected cell microarrays; affinity-based arrays; cell attachment and stimulation; 3D cell constructs; microfluidic devices; organ-on-a-chip (OOC) living cell microarrays (LCMAs); transfected cell microarrays; affinity-based arrays; cell attachment and stimulation; 3D cell constructs; microfluidic devices; organ-on-a-chip (OOC)
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Jonczyk, R.; Kurth, T.; Lavrentieva, A.; Walter, J.-G.; Scheper, T.; Stahl, F. Living Cell Microarrays: An Overview of Concepts. Microarrays 2016, 5, 11.

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