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From the third issue of 2017, Microarrays has changed its name to High-Throughput.

Open AccessArticle
Microarrays 2016, 5(2), 8; doi:10.3390/microarrays5020008

Time-Resolved Study of Nanoparticle Induced Apoptosis Using Microfabricated Single Cell Arrays

1
Faculty of Physics and Center for NanoSciene (CeNS), Ludwig-Maximilians-Universität, Geschwister-Scholl-Platz 1, 80539 Munich, Germany
2
Centre for BioNano Interactions, School of Chemistry and Chemical Biology, University College Dublin, Belfield, Dublin 4, Ireland
*
Author to whom correspondence should be addressed.
Academic Editor: Holger Erfle
Received: 3 March 2016 / Revised: 1 April 2016 / Accepted: 7 April 2016 / Published: 15 April 2016
(This article belongs to the Special Issue Cell-Based Microarrays)
View Full-Text   |   Download PDF [4826 KB, uploaded 15 April 2016]   |  

Abstract

Cell fate decisions like apoptosis are heterogeneously implemented within a cell population and, consequently, the population response is recognized as sum of many individual dynamic events. Here, we report on the use of micro-patterned single-cell arrays for real-time tracking of nanoparticle-induced (NP) cell death in sets of thousands of cells in parallel. Annexin (pSIVA) and propidium iodide (PI), two fluorescent indicators of apoptosis, are simultaneously monitored after exposure to functionalized polystyrene (PS NH 2) nanobeads as a model system. We find that the distribution of Annexin onset times shifts to later times and broadens as a function of decreasing NP dose. We discuss the mean time-to-death as a function of dose, and show how the EC 50 value depends both on dose and time of measurement. In addition, the correlations between the early and late apoptotic markers indicate a systematic shift from apoptotic towards necrotic cell death during the course of the experiment. Thus, our work demonstrates the potential of array-based single cell cytometry for kinetic analysis of signaling cascades in a high-throughput format. View Full-Text
Keywords: single cell array; cytotoxicity; nanoparticle; dose-response; correlation studies; micropatterning single cell array; cytotoxicity; nanoparticle; dose-response; correlation studies; micropatterning
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Röttgermann, P.J.F.; Dawson, K.A.; Rädler, J.O. Time-Resolved Study of Nanoparticle Induced Apoptosis Using Microfabricated Single Cell Arrays. Microarrays 2016, 5, 8.

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