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Microarrays 2013, 2(2), 63-80; doi:10.3390/microarrays2020063
Article

Phenotypic MicroRNA Microarrays

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Received: 8 February 2013; in revised form: 20 March 2013 / Accepted: 25 March 2013 / Published: 3 April 2013
(This article belongs to the Special Issue MicroRNA Microarrays)
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Abstract: Microarray technology has become a very popular approach in cases where multiple experiments need to be conducted repeatedly or done with a variety of samples. In our lab, we are applying our high density spots microarray approach to microscopy visualization of the effects of transiently introduced siRNA or cDNA on cellular morphology or phenotype. In this publication, we are discussing the possibility of using this micro-scale high throughput process to study the role of microRNAs in the biology of selected cellular models. After reverse-transfection of microRNAs and siRNA, the cellular phenotype generated by microRNAs regulated NF-κB expression comparably to the siRNA. The ability to print microRNA molecules for reverse transfection into cells is opening up the wide horizon for the phenotypic high content screening of microRNA libraries using cellular disease models.
Keywords: microRNA; siRNA; phenotypic screen microRNA; siRNA; phenotypic screen
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Kwon, Y.-J.; Heo, J.Y.; Kim, H.C.; Kim, J.Y.; Liuzzi, M.; Soloveva, V. Phenotypic MicroRNA Microarrays. Microarrays 2013, 2, 63-80.

AMA Style

Kwon Y-J, Heo JY, Kim HC, Kim JY, Liuzzi M, Soloveva V. Phenotypic MicroRNA Microarrays. Microarrays. 2013; 2(2):63-80.

Chicago/Turabian Style

Kwon, Yong-Jun; Heo, Jin Y.; Kim, Hi C.; Kim, Jin Y.; Liuzzi, Michel; Soloveva, Veronica. 2013. "Phenotypic MicroRNA Microarrays." Microarrays 2, no. 2: 63-80.


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