Next Article in Journal
Synthetic Cannabinoid use in a Case Series of Patients with Psychosis Presenting to Acute Psychiatric Settings: Clinical Presentation and Management Issues
Previous Article in Journal
Perceived Discrimination and Substance Use among Caribbean Black Youth; Gender Differences
Article Menu
Issue 7 (July) cover image

Export Article

Open AccessArticle
Brain Sci. 2018, 8(7), 132; https://doi.org/10.3390/brainsci8070132

A Multidisciplinary Approach Reveals an Age-Dependent Expression of a Novel Bioactive Peptide, Already Involved in Neurodegeneration, in the Postnatal Rat Forebrain

1
Neuro-Bio Ltd., Culham Science Centre, Building F5, Abingdon OX14 3DB, UK
2
School of Physiology, Pharmacology and Neuroscience, Faculty of Biomedical Sciences, University of Bristol, Bristol BS8 1TD, UK
3
School of Life Sciences, University of Warwick, Coventry CV4 7AL, UK
These authors contributed equally to this work.
*
Authors to whom correspondence should be addressed.
Received: 29 May 2018 / Revised: 4 July 2018 / Accepted: 6 July 2018 / Published: 10 July 2018
Full-Text   |   PDF [1789 KB, uploaded 10 July 2018]   |  

Abstract

The basal forebrain has received much attention due to its involvement in multiple cognitive functions, but little is known about the basic neuronal mechanisms underlying its development, nor those mediating its primary role in Alzheimer’s disease. We have previously suggested that a novel 14-mer peptide, ‘T14’, could play a pivotal role in Alzheimer’s disease, via reactivation of a developmental signaling pathway. In this study, we have characterized T14 in the context of post-natal rat brain development, using a combination of different techniques. Ex-vivo rat brain slices containing the basal forebrain, at different stages of development, were used to investigate large-scale neuronal network activity in real time with voltage-sensitive dye imaging. Subsequent Western blot analysis revealed the expression profile of endogenous T14, its target alpha7 nicotinic receptor and the familiar markers of Alzheimer’s: amyloid beta and phosphorylated Tau. Results indicated maximal neuronal activity at the earliest ages during development, reflected in a concomitant profile of T14 peptide levels and related proteins. In conclusion, these findings show that the peptide, already implicated in neurodegenerative events, has an age-dependent expression, suggesting a possible contribution to the physiological mechanisms underlying brain maturation. View Full-Text
Keywords: development; basal forebrain; AChE-derived peptide; nicotinic receptors; optical imaging; Alzheimer’s disease development; basal forebrain; AChE-derived peptide; nicotinic receptors; optical imaging; Alzheimer’s disease
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Supplementary material

SciFeed

Share & Cite This Article

MDPI and ACS Style

Ferrati, G.; Brai, E.; Stuart, S.; Marino, C.; Greenfield, S.A. A Multidisciplinary Approach Reveals an Age-Dependent Expression of a Novel Bioactive Peptide, Already Involved in Neurodegeneration, in the Postnatal Rat Forebrain. Brain Sci. 2018, 8, 132.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Brain Sci. EISSN 2076-3425 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top