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Appl. Sci. 2017, 7(3), 242; doi:10.3390/app7030242

Synthesis and Physical Properties of Polyhydroxyalkanoate Polymers with Different Monomer Compositions by Recombinant Pseudomonas putida LS46 Expressing a Novel PHA SYNTHASE (PhaC116) Enzyme

1
Department of Biosystems Engineering, University of Manitoba, Winnipeg, MB R3T 5V6, Canada
2
Department of Biology and Centre for Bioengineering and Biotechnology, University of Waterloo, Waterloo, ON N2L 3G1, Canada
*
Author to whom correspondence should be addressed.
Academic Editor: Chih-Ching Huang
Received: 23 December 2016 / Revised: 29 January 2017 / Accepted: 23 February 2017 / Published: 3 March 2017
(This article belongs to the Special Issue Polyhydroxyalkanoates and Their Applications)
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Abstract

A recombinant of Pseudomonas putida LS461 (deletion of the phaC1phaZphaC2 genes) was constructed by introducing cosmid JC123 carrying a novel phaC116 gene from a metagenomic clone. The resulting strain, P. putida LS46123, was able to synthesize polyhydroxyalkanoate (PHA) polymers with novel monomer compositions when cultured on glucose or free fatty acids, and accumulated PHAs from 9.24% to 27.09% of cell dry weight. The PHAs synthesized by P. putida LS46123 contained up to 50 mol % short chain length subunits (3-hydroxybutyrate and 3-hydroxyvalerate), with the remaining monomers consisting of various medium chain length subunits. The PhaC116 protein expressed by P. putida LS46123 had an amino acid sequence similarity of 45% with the PhaC1 protein of the parent strain, P. putida LS46. Predicted 3D structures of the PhaC116 proteins from P. putida LS46123 and P. putida LS46 revealed several differences in the numbers and locations of protein secondary structures. The physical and thermal properties of the novel polymers synthesized by P. putida LS46123 cultured with glucose or free fatty acids differed significantly from those produced by P. putida LS46 grown on the same substrates. PHA polymers with different subunit compositions, and hence different physical and thermal properties, can be tailor-made using novel PHA synthase for specific applications. View Full-Text
Keywords: Pseudomonas putida; polyhydroxyalkanoates; PHA synthase; copolymers; physical properties of PHA polymers Pseudomonas putida; polyhydroxyalkanoates; PHA synthase; copolymers; physical properties of PHA polymers
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MDPI and ACS Style

Sharma, P.K.; Munir, R.I.; Blunt, W.; Dartiailh, C.; Cheng, J.; Charles, T.C.; Levin, D.B. Synthesis and Physical Properties of Polyhydroxyalkanoate Polymers with Different Monomer Compositions by Recombinant Pseudomonas putida LS46 Expressing a Novel PHA SYNTHASE (PhaC116) Enzyme. Appl. Sci. 2017, 7, 242.

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