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Appl. Sci. 2017, 7(3), 225; doi:10.3390/app7030225

Enhanced Agarose and Xylan Degradation for Production of Polyhydroxyalkanoates by Co-Culture of Marine Bacterium, Saccharophagus degradans and Its Contaminant, Bacillus cereus

1
Department of Chemical Engineering, Chungbuk National University, Cheongju, Chungbuk 28644, Korea
2
Biosciences Center, National Renewable Energy Laboratory, Golden, CO 80401, USA
*
Author to whom correspondence should be addressed.
Academic Editor: Ipsita Roy
Received: 21 December 2016 / Revised: 31 January 2017 / Accepted: 2 February 2017 / Published: 28 February 2017
(This article belongs to the Special Issue Polyhydroxyalkanoates and Their Applications)
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Abstract

Over reliance on energy or petroleum products has raised concerns both in regards to the depletion of their associated natural resources as well as their increasing costs. Bioplastics derived from microbes are emerging as promising alternatives to fossil fuel derived petroleum plastics. The development of a simple and eco-friendly strategy for bioplastic production with high productivity and yield, which is produced in a cost effective manner utilising abundantly available renewable carbon sources, would have the potential to result in an inexhaustible global energy source. Here we report the biosynthesis of bioplastic polyhydroxyalkanoates (PHAs) in pure cultures of marine bacterium, Saccharophagus degradans 2-40 (Sde 2-40), its contaminant, Bacillus cereus, and a co-culture of these bacteria (Sde 2-40 and B. cereus) degrading plant and algae derived complex polysaccharides. Sde 2-40 degraded the complex polysaccharides agarose and xylan as sole carbon sources for biosynthesis of PHAs. The ability of Sde 2-40 to degrade agarose increased after co-culturing with B. cereus. The association of Sde 2-40 with B. cereus resulted in increased cell growth and higher PHA production (34.5% of dry cell weight) from xylan as a carbon source in comparison to Sde 2-40 alone (22.7% of dry cell weight). The present study offers an innovative prototype for production of PHA through consolidated bioprocessing of complex carbon sources by pure and co-culture of microorganisms. View Full-Text
Keywords: polyhydroxyalkanoates; agarose; xylan; Saccharophagus degradans; Bacillus cereus; consolidated bioprocessing polyhydroxyalkanoates; agarose; xylan; Saccharophagus degradans; Bacillus cereus; consolidated bioprocessing
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MDPI and ACS Style

Sawant, S.S.; Salunke, B.K.; Taylor, L.E.; Kim, B.S. Enhanced Agarose and Xylan Degradation for Production of Polyhydroxyalkanoates by Co-Culture of Marine Bacterium, Saccharophagus degradans and Its Contaminant, Bacillus cereus. Appl. Sci. 2017, 7, 225.

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