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Appl. Sci. 2017, 7(10), 1041; doi:10.3390/app7101041

Interactions of Insolation and Shading on Ability to Use Fluorescence Imaging to Detect Fecal Contaminated Spinach

1
USDA (United States Department of Agriculture) Agricultural Research Service, Rm 21, Bldg 303, BARC-East, Powder Mill Rd., Beltsville, MD 20705, USA
2
Department of Agricultural and Biosystems Engineering, Yuma Agricultural Center, University of Arizona, 6425 W. 8th St., Yuma, AZ 85364, USA
*
Author to whom correspondence should be addressed.
Received: 29 September 2017 / Accepted: 10 October 2017 / Published: 12 October 2017
(This article belongs to the Special Issue Hyperspectral Chemical Imaging for Food Authentication)
View Full-Text   |   Download PDF [11862 KB, uploaded 12 October 2017]   |  

Abstract

Fecal contamination of produce in fields is a recognized food safety risk, and it is a requirement that fields be surveyed for evidence of fecal contamination. It may be possible to increase the efficacy of such surveys using imaging techniques that rely on detection of fluorescence responses of fecal material to UV excitation. However, fluorescence responses are easily masked by ambient illumination. This study investigated the potential of using a shroud to reduce the impact of ambient illumination on responses measured using relatively inexpensive optical components. During periods of near peak insolation, even with full shrouding, results indicate that reliable detection would be problematic. Towards dusk, effective imaging could be accomplished even with a gap of 250 cm at the bottom of the shroud. Results suggest that imaging using relatively inexpensive components could provide the basis for detection of fecal contamination in produce fields if surveys were conducted during dawn or dusk, or at night. View Full-Text
Keywords: imaging; hyperspectral imaging; fluorescence imaging; food safety; leafy greens imaging; hyperspectral imaging; fluorescence imaging; food safety; leafy greens
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Lefcourt, A.M.; Siemens, M.C. Interactions of Insolation and Shading on Ability to Use Fluorescence Imaging to Detect Fecal Contaminated Spinach. Appl. Sci. 2017, 7, 1041.

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