Next Article in Journal
An Efficient Causal Group Communication Protocol for Free Scale Peer-to-Peer Networks
Previous Article in Journal
L1 Adaptive Control for a Vertical Rotor Orientation System
Article Menu

Export Article

Open AccessArticle
Appl. Sci. 2016, 6(9), 244; doi:10.3390/app6090244

Application of Response Surface Methodology for Optimisation of Simultaneous UHPLC-PDA Determination of Oleanolic and Ursolic Acids and Standardisation of Ericaceae Medicinal Plants

1
Department of Pharmacognosy, Faculty of Pharmacy, Medical University of Lodz, 1 Muszynskiego St., Lodz 90-151, Poland
2
Department of Biology and Pharmaceutical Botany, Faculty of Pharmacy, Medical University of Lodz, 1 Muszynskiego St., Lodz 90-151, Poland
*
Author to whom correspondence should be addressed.
Academic Editor: Hidenori Otsuka
Received: 5 July 2016 / Revised: 13 August 2016 / Accepted: 24 August 2016 / Published: 29 August 2016
(This article belongs to the Section Chemistry)
View Full-Text   |   Download PDF [2348 KB, uploaded 29 August 2016]   |  

Abstract

A fast and sensitive ultra-high performance liquid chromatography-photodiode array (UHPLC-PDA) method for simultaneous quantification of oleanolic acid (OA) and ursolic acid (UA) in plant materials was developed. A central composite design combined with a response surface methodology was utilized to establish optimal separation conditions. The final separation was accomplished on a Zorbax Eclipse XDB-C18 column (1.8 μm, 100 mm × 3 mm I.D., Agilent, Santa Clara, CA, USA) using a mixture 90:10 (v/v) of methanol and 1% (w/v) aqueous orthophosporic acid as a mobile phase at a flow rate of 0.44 mL/min and temperature of 18 °C. The analysis was completed in 6.2 min with satisfactory resolution of 1.5 between the target analytes. The developed method proved to be precise (relative standard deviations below 3.2%), accurate (recoveries in the range of 95.27%–98.60%), and sensitive (limits of detection (LODs) in the range of 0.047–0.051 mg/mL). The method was then successfully applied to evaluate OA and UA content in real samples of selected Ericaceae plant materials (leaves of Arctostaphylos uva ursi, Vaccinium myrtillus, Vaccinium vitis idaea, Gaultheria procumbens). The content of OA and UA in investigated samples varied in the range of 0.74–4.47 mg/g dry weight (dw) and 1.30–18.61 mg/g dw, respectively. View Full-Text
Keywords: oleanolic acid; ursolic acid; response surface methodology; Ericaceae; Vaccinium vitis idaea; Vaccinium myrtillus; Arctostaphylos uva ursi; Gaultheria procumbens; triterpenoic acids oleanolic acid; ursolic acid; response surface methodology; Ericaceae; Vaccinium vitis idaea; Vaccinium myrtillus; Arctostaphylos uva ursi; Gaultheria procumbens; triterpenoic acids
Figures

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Owczarek, A.; Kuźma, Ł.; Wysokińska, H.; Olszewska, M.A. Application of Response Surface Methodology for Optimisation of Simultaneous UHPLC-PDA Determination of Oleanolic and Ursolic Acids and Standardisation of Ericaceae Medicinal Plants. Appl. Sci. 2016, 6, 244.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Appl. Sci. EISSN 2076-3417 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top