Next Article in Journal
Photoacoustic Tomography Imaging of the Adult Zebrafish by Using Unfocused and Focused High-Frequency Ultrasound Transducers
Previous Article in Journal
Optimization of Silicon MZM Fabrication Parameters for High Speed Short Reach Interconnects at 1310 nm
Article Menu

Export Article

Open AccessArticle
Appl. Sci. 2016, 6(12), 394; doi:10.3390/app6120394

Detection of Proteins Using Nano Magnetic Particle Accumulation-Based Signal Amplification

1
BioMINDS (Bio Micro/Nano Devices and Sensors) Lab, Department of Electrical and Electronics Engineering, Abdullah Gül University, Kayseri 38080, Turkey
2
Bioengineering Department, Abdullah Gül University, Kayseri 38080, Turkey
*
Author to whom correspondence should be addressed.
Academic Editor: Raed Abu-Reziq
Received: 4 November 2016 / Revised: 22 November 2016 / Accepted: 24 November 2016 / Published: 29 November 2016
(This article belongs to the Section Nanotechnology and Applied Nanosciences)
View Full-Text   |   Download PDF [2411 KB, uploaded 29 November 2016]   |  

Abstract

We report a biosensing method based on magnetic particles where coated magnetic particles are used for immunomagnetic separation, and uncoated magnetic particles are used for signal enhancement. To quantify the signal amplification, optical micrographs are analyzed to measure changes in pixel area and pixel intensity. Microcontact-printed surface receptors are arranged in alternating lines on gold chips, enabling differential calculations. In a model experiment, target molecules-streptavidin-are first captured and separated by biotin-coated magnetic particles, and then exposed to a gold surface functionalized with biotin-coupled bovine serum albumin, forming a sandwich assay. Applying a magnetic field and introducing uncoated magnetic particles resulted in accumulation around magnetic particles in the sandwich assay and enhancement of the contrast to noise ratio at least by eight-fold in a range of 0.1–100 μM. View Full-Text
Keywords: magnetic nano particle; magnetic accumulation; contrast-to-noise ratio; micro contact printing; biosensing; signal amplification magnetic nano particle; magnetic accumulation; contrast-to-noise ratio; micro contact printing; biosensing; signal amplification
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Supplementary material

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

İçöz, K.; Mzava, O. Detection of Proteins Using Nano Magnetic Particle Accumulation-Based Signal Amplification. Appl. Sci. 2016, 6, 394.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Appl. Sci. EISSN 2076-3417 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top