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Appl. Sci. 2016, 6(11), 328; doi:10.3390/app6110328

Low-Cost Temperature Logger for a Polymerase Chain Reaction Thermal Cycler

1
Department of Convergence Software, Hallym University, Chuncheon 24252, Korea
2
Bio-IT Research Center, Hallym University, Chuncheon 24252, Korea
*
Author to whom correspondence should be addressed.
Academic Editor: Wen-Hsiang Hsieh
Received: 13 September 2016 / Revised: 27 October 2016 / Accepted: 27 October 2016 / Published: 31 October 2016
View Full-Text   |   Download PDF [1656 KB, uploaded 31 October 2016]   |  

Abstract

Polymerase chain reaction (PCR) is a method of amplifying DNA which is normally carried out with a thermal cycler. To obtain more accurate and reliable PCR results, the temperature change within the chamber of the thermal cycler needs to be verified and calibrated regularly. Commercially available temperature loggers commonly used for temperature verification tests usually require a graphical user interface (GUI) attached to the logger for convenience and straightforward understanding of the device. In this study, a host-local architecture for the temperature logger that significantly reduces the development time and cost is proposed. Employing standard computing devices as the host gives better development environment and user-friendly GUI. This paper presents the hardware and software design of the host-local temperature logger, and demonstrates the use of the local temperature logger connected to a personal computer with a Windows operating system. The probe design, thermistor resistance measurement, temperature filtering, and temperature calibration is described in detail. The thermistor self-heating problem was investigated in particular to determine the reference resistor that was serially connected to the thermistor. The temperature accuracy and temporal precision of the proposed system was 0.1 K. View Full-Text
Keywords: PCR; the negative temperature coefficient (NTC) thermistor; GUI; calibration; filtering; log; self-heating PCR; the negative temperature coefficient (NTC) thermistor; GUI; calibration; filtering; log; self-heating
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Park, C.-Y.; Cho, J.-H.; Kim, Y.-S.; Song, H.-J.; Kim, J.-D. Low-Cost Temperature Logger for a Polymerase Chain Reaction Thermal Cycler. Appl. Sci. 2016, 6, 328.

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