Next Article in Journal
Three-Phase PV CHB Inverter for a Distributed Power Generation System
Previous Article in Journal
A Novel Prosumer-Based Energy Sharing and Management (PESM) Approach for Cooperative Demand Side Management (DSM) in Smart Grid
Article Menu

Export Article

Open AccessArticle
Appl. Sci. 2016, 6(10), 286; doi:10.3390/app6100286

Classical Optimization of Cellulase and Xylanase Production by a Marine Streptomyces Species

1
Applied and Environmental Microbiology Research Group, Department of Biochemistry and Microbiology, University of Fort Hare, Alice 5700, South Africa
2
SAMRC Microbial Water Quality Monitoring Centre, University of Fort Hare, Alice 5700, South Africa
*
Author to whom correspondence should be addressed.
Academic Editor: Chih-Ching Huang
Received: 21 July 2016 / Revised: 21 September 2016 / Accepted: 27 September 2016 / Published: 9 October 2016
View Full-Text   |   Download PDF [1244 KB, uploaded 9 October 2016]   |  

Abstract

Cellulase and xylanase are in high demand for application in several industrial processes, consequently necessitating the bioprospecting and manipulation of microbes for novel and greater enzyme productivity. This study reports on the optimal conditions for cellulase and xylanase production by a marine bacterial isolate from Nahoon beach sediment, via the classical process of one variable per time. Furthermore, the inducing effect of mono- and polysaccharides on enzyme production was investigated. The 16S rDNA gene sequence analysis clearly assigned the isolate to the genus Streptomyces, and was deposited at the GenBank under the accession number KU171373. Cellulase and xylanase production was optimal at the following conditions: pH 6 and 8, incubation temperature of 40 and 35 °C, and agitation speed of 100 and 150 rpm, respectively. Under optimum conditions, 0.26 U/mL and 18.54 U/mL activities were attained at 60 and 48 h with specific productivity of 205 and 7417 U/g for cellulase and xylanase, respectively. Xylanase production was induced by the entire mono- and polysaccharides tested, while cellulase production was induced by some. The results from this study signify the resourcefulness of the Streptomyces strain for production of cellulase and xylanase of industrial importance. View Full-Text
Keywords: cellulase; xylanase; optimization; saccharides; Streptomyces; activity; production cellulase; xylanase; optimization; saccharides; Streptomyces; activity; production
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Fatokun, E.N.; Nwodo, U.U.; Okoh, A.I. Classical Optimization of Cellulase and Xylanase Production by a Marine Streptomyces Species. Appl. Sci. 2016, 6, 286.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Appl. Sci. EISSN 2076-3417 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top