Appl. Sci. 2012, 2(4), 754-772; doi:10.3390/app2040754
Article

Use of Residual Biomass from the Textile Industry as Carbon Source for Production of a Low-Molecular-Weight Xylanase from Aspergillus oryzae

1 Laboratory of Genetics and Biochemistry, Biological Sciences Course, Federal University of Tocantins, Porto Nacional, TO, CEP 77500-000, Brazil 2 Laboratory of Enzymology, Department of Cellular Biology, University of Brasília, Brasília, DF, CEP 70910-900, Brazil 3 Laboratory of Biochemistry and Protein Chemistry, Department of Cellular Biology, University of Brasília, Brasília, DF, CEP 70910-900, Brazil 4 Laboratory of Industrial Microbiology and Enzymology, Multidisciplinary Institute for Health, Federal University of Bahia, Vitoria da Conquista, BA, CEP 45029-094, Brazil 5 Laboratory of Food Microbiology, Department of Food Science, Federal University of Lavras, Lavras, MG, CEP 37200-000, Brazil 6 Laboratory of Plant Physiology, Department of Botany, University of Brasília, Brasília, DF, CEP 70910-900, Brazil
* Author to whom correspondence should be addressed.
Received: 18 September 2012; in revised form: 12 October 2012 / Accepted: 12 October 2012 / Published: 23 October 2012
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Abstract: Pretreated dirty cotton residue (PDCR) from the textile industry was used as an alternative carbon source for the submerged cultivation of Aspergillus oryzae and the production of xylanases. The filtered culture supernatant was fractionated by ultrafiltration followed by three chromatographic steps, which resulted in the isolation of a homogeneous low-molecular-weight xylanase (Xyl-O1) with a mass of 21.5 kDa as determined by sodium dodecyl sulfate-polyacrilamide gel electrophoresis (SDS-PAGE) co-polymerized with 0.1% oat spelt xylan. Enzyme catalysis was the most efficient at 50 °C and pH 6.0. The Km values (mg·mL−1) for the soluble fraction of oat spelt and birchwood xylans were 10.05 and 3.34, respectively. Xyl-O1 was more stable in the presence of 5,5-dithio-bis-(2-nitrobenzoic acid) (DTNB), 1,4-dithiothreitol (DTT), l-cysteine or β-mercaptoethanol, which increased the rate of catalysis by 40%, 14%, 40% or 37%, respectively. The enzyme stability was improved at pH 7.0 in the presence of 20 mM l-cysteine, with the retention of nearly 100% of the activity after 6 h at 50 °C. Xyl-O1 catalyzed the cleavage of internal β-1,4 linkages of the soluble substrates containing d-xylose residues, with a maximum efficiency of 33% for the hydrolysis of birchwood xylan after 12 h of incubation. Identification of the hydrolysis products by high-performance anion exchange chromatography coupled with pulsed amperometric detection (HPAEC-PAD) indicated the predominance of the hydrolysis products X2-X6 during the first 12 h of incubation and the accumulation of higher xylooligomers after the elution of the last xylooligomer standard, xylohexaose.
Keywords: agro-industrial residues; hemicellulose; enzyme purification; xylanase; hydrolysis

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MDPI and ACS Style

Duarte, G.C.; Moreira, L.R.S.; Gómez-Mendoza, D.P.; Siqueira, F.G.; Batista, L.R.; Amaral, L.I.V.; Ricart, C.A.O.; Filho, E.X.F. Use of Residual Biomass from the Textile Industry as Carbon Source for Production of a Low-Molecular-Weight Xylanase from Aspergillus oryzae. Appl. Sci. 2012, 2, 754-772.

AMA Style

Duarte GC, Moreira LRS, Gómez-Mendoza DP, Siqueira FG, Batista LR, Amaral LIV, Ricart CAO, Filho EXF. Use of Residual Biomass from the Textile Industry as Carbon Source for Production of a Low-Molecular-Weight Xylanase from Aspergillus oryzae. Applied Sciences. 2012; 2(4):754-772.

Chicago/Turabian Style

Duarte, Gilvan C.; Moreira, Leonora R.S.; Gómez-Mendoza, Diana P.; Siqueira, Félix Gonçalves de; Batista, Luís R.; Amaral, Lourdes I.V.; Ricart, Carlos A.O.; Filho, Edivaldo X.F. 2012. "Use of Residual Biomass from the Textile Industry as Carbon Source for Production of a Low-Molecular-Weight Xylanase from Aspergillus oryzae." Appl. Sci. 2, no. 4: 754-772.

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