Next Article in Journal
Healthcare Cost and Utilization before and after Diagnosis of Pseudomonas aeruginosa among Patients with Non-Cystic Fibrosis Bronchiectasis in the U.S.
Next Article in Special Issue
Cytokine Signaling in Multiple Sclerosis and Its Therapeutic Applications
Previous Article in Journal
The Association between Sleep and Theory of Mind in School Aged Children with ADHD
Article Menu

Export Article

Open AccessFeature PaperCommunication
Med. Sci. 2017, 5(3), 19; doi:10.3390/medsci5030019

Molecular Characterization of Peripheral Extracellular Vesicles in Clinically Isolated Syndrome: Preliminary Suggestions from a Pilot Study

1
National Research Council of Italy, Institute of Biomedical Technologies, Section of Bari, via Amendola no. 122/D, 70126 Bari, Italy
2
Department of Basic Sciences, Neurosciences and Sense Organs, University of Bari, 70126 Bari, Italy
3
Department of Medical and Surgical Sciences, University of Foggia, 71121 Foggia, Italy
*
Author to whom correspondence should be addressed.
Received: 21 August 2017 / Revised: 7 September 2017 / Accepted: 14 September 2017 / Published: 18 September 2017
(This article belongs to the Special Issue Advances in the Pathogenesis of Neurodegenerative Diseases)
View Full-Text   |   Download PDF [536 KB, uploaded 18 September 2017]   |  

Abstract

Extracellular vesicles (EVs), nanoparticles originated from different cell types, seem to be implicated in several cellular activities. In the Central Nervous System (CNS), glia and neurons secrete EVs and recent studies have demonstrated that the intercellular communication mediated by EVs has versatile functional impact in the cerebral homeostasis. This essential role may be due to their proteins and RNAs cargo that possibly modify the phenotypes of the targeted cells. Despite the increasing importance of EVs, little is known about their fluctuations in physiological as well as in pathological conditions. Furthermore, only few studies have investigated the contents of contemporary EVs subgroups (microvesicles, MVs and exosomes, EXOs) with the purpose of discriminating between their features and functional roles. In order to possibly shed light on these issues, we performed a pilot study in which MVs and EXOs extracted from serum samples of a little cohort of subjects (patients with the first clinical evidence of CNS demyelination, also known as Clinically Isolated Syndrome and Healthy Controls) were submitted to deep small-RNA sequencing. Data were analysed by an in-home bioinformatics platform. In line with previous reports, distinct classes of non-coding RNAs have been detected in both the EVs subsets, offering interesting suggestions on their origins and functions. We also verified the feasibility of this extensive molecular approach, thus supporting its valuable use for the analysis of circulating biomarkers (e.g., microRNAs) in order to investigate and monitor specific diseases. View Full-Text
Keywords: extracellular vesicles (EVs); Clinically Isolated Syndrome (CIS); small RNA sequencing; circulating biomarkers; bioinformatics extracellular vesicles (EVs); Clinically Isolated Syndrome (CIS); small RNA sequencing; circulating biomarkers; bioinformatics
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Supplementary material

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Nuzziello, N.; Blonda, M.; Licciulli, F.; Liuni, S.; Amoruso, A.; Valletti, A.; Consiglio, A.; Avolio, C.; Liguori, M. Molecular Characterization of Peripheral Extracellular Vesicles in Clinically Isolated Syndrome: Preliminary Suggestions from a Pilot Study. Med. Sci. 2017, 5, 19.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Med. Sci. EISSN 2076-3271 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top