New 19-Residue Peptaibols from Trichoderma Clade Viride
AbstractTrichoderma koningiopsis and T. gamsii belong to clade Viride of Trichoderma, the largest and most diverse group of this genus. They produce a wide range of bioactive secondary metabolites, including peptaibols with antibacterial, antifungal, and antiviral properties. The unusual amino acid residues of peptaibols, i.e., α-aminoisobutyric acid (Aib), isovaline (Iva), and the C-terminal 1,2-amino alcohol make them unique among peptides. In this study, the peptaibiomes of T. koningiopsis and T. gamsii were investigated by HPLC-ESI-MS. The examined strains appeared to produce 19-residue peptaibols, most of which are unknown from literature, but their amino acid sequences are similar to those of trikoningins, tricholongins, trichostrigocins, trichorzianins, and trichorzins. A new group of peptaibols detected in T. koningiopsis are described here under the name “Koningiopsin”. Trikoningin KA V, the closest peptaibol compound to the peptaibols produced by these two strains, was selected for structural investigation by short MD simulation, which revealed that many residues show high preference for left handed helix formation. The bioactivity of the peptaibol mixtures produced by T. koningiopsis and T. gamsii was tested on agar plates against bacteria, yeasts, and filamentous fungi. The results revealed characteristic differences in bioactivities towards the different groups of target microorganisms, which can be explained with the differences in their cell wall structures. View Full-Text
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Marik, T.; Tyagi, C.; Racić, G.; Rakk, D.; Szekeres, A.; Vágvölgyi, C.; Kredics, L. New 19-Residue Peptaibols from Trichoderma Clade Viride. Microorganisms 2018, 6, 85.
Marik T, Tyagi C, Racić G, Rakk D, Szekeres A, Vágvölgyi C, Kredics L. New 19-Residue Peptaibols from Trichoderma Clade Viride. Microorganisms. 2018; 6(3):85.Chicago/Turabian Style
Marik, Tamás; Tyagi, Chetna; Racić, Gordana; Rakk, Dávid; Szekeres, András; Vágvölgyi, Csaba; Kredics, László. 2018. "New 19-Residue Peptaibols from Trichoderma Clade Viride." Microorganisms 6, no. 3: 85.
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