Next Article in Journal
EPS—Then and Now
Next Article in Special Issue
Improvement of Intestinal Immune Cell Function by Lactic Acid Bacteria for Dairy Products
Previous Article in Journal
Natural Products for the Treatment of Chlamydiaceae Infections
Previous Article in Special Issue
Adhesion Properties of Lactic Acid Bacteria on Intestinal Mucin
Article Menu

Export Article

Open AccessArticle
Microorganisms 2016, 4(4), 40; doi:10.3390/microorganisms4040040

Human Lactobacillus Strains from the Intestine can Suppress IgE-Mediated Degranulation of Rat Basophilic Leukaemia (RBL-2H3) Cells

1
Technical Research Laboratory, Takanashi Milk Products Co., Ltd., Yokohama 241-0023, Japan
2
Department of Food Bioscience and Biotechnology, College of Bioresource Sciences, Nihon University, Fujisawa 252-8510, Japan
*
Author to whom correspondence should be addressed.
Academic Editors: Haruki Kitazawa and Julio Villena
Received: 15 August 2016 / Revised: 7 October 2016 / Accepted: 24 October 2016 / Published: 27 October 2016
(This article belongs to the Special Issue Probiotic Microorganisms: An Intimate Gaze)
View Full-Text   |   Download PDF [1986 KB, uploaded 27 October 2016]   |  

Abstract

Mast cells play a critical role in immunoglobulin E (IgE)-mediated allergic diseases, and the degranulation of mast cells is important in the pathogenesis of these diseases. A disturbance of the intestinal microflora, especially of endogenous lactic acid bacteria, might be a contributing factor for IgE-mediated allergic diseases. Additional knowledge regarding the interaction of human intestinal Lactobacilli with mast cells is still necessary. Twenty-three strains of Lactobacilli, including commercial and reference strains and strains from the human intestine, were tested for their ability to regulate degranulation of cells from rat basophilic leukemia RBL-2H3 cells (RBL-2H3) in vitro based on a β-hexosaminidase release assay. Each of the tested Lactobacilli characteristically suppressed IgE-mediated degranulation of RBL-2H3 cells, and Lactobacillus GG showed the strongest inhibitory effect on the cells. Furthermore, the bacteria isolated from the human intestine significantly suppressed degranulation of RBL-2H3 cellsin comparison with the reference strains. These results suggest that Lactobacilli, particularly those from the human intestine, can affect the activation of mast cells in a strain-dependent manner. Further study should be conducted to analyse the understanding mechanism. View Full-Text
Keywords: degranulation; IgE-mediated allergy; Lactobacilli; mast cell degranulation; IgE-mediated allergy; Lactobacilli; mast cell
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Harata, G.; He, F.; Takahashi, K.; Hosono, A.; Miyazawa, K.; Yoda, K.; Hiramatsu, M.; Kaminogawa, S. Human Lactobacillus Strains from the Intestine can Suppress IgE-Mediated Degranulation of Rat Basophilic Leukaemia (RBL-2H3) Cells. Microorganisms 2016, 4, 40.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Microorganisms EISSN 2076-2607 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top