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Pathogens 2017, 6(4), 45; https://doi.org/10.3390/pathogens6040045

The Use of Hyperimmune Chicken Reference Sera Is Not Appropriate for the Validation of Influenza Pseudotype Neutralization Assays

1
Viral Pseudotype Unit, Medway School of Pharmacy, University of Kent, Chatham, Kent ME4 4TB, UK
2
Animal Production and Health Laboratory, Joint FAO/IAEA Division, International Atomic Energy Agency, A-2444 Seibersdorf, Austria
*
Author to whom correspondence should be addressed.
Received: 23 August 2017 / Revised: 13 September 2017 / Accepted: 20 September 2017 / Published: 25 September 2017
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Abstract

The pseudotype particle neutralization test (pp-NT) is a next-generation serological assay employed for the sensitive study of influenza antibody responses against hemagglutinin (HA), including stalk-directed antibodies. However, a validation of this assay has yet to be performed, and this limits its use to primarily research laboratories. To identify possible serological standards to be used in optimization and validation of the pp-NT, we have evaluated the cross-reactivity of hyperimmune chicken reference antisera in this assay. Our findings show that the cross-reactivity detected by the pp-NT is only partly explained by phylogenetic relationships and protein homology between the HA subtypes analysed; further studies are necessary to understand the origin of the cross-reactivity detected, and reference standards with higher specificity should be evaluated or generated de novo for future use in pp-NT. View Full-Text
Keywords: influenza; serology; standards; validation; pseudotypes; neutralization influenza; serology; standards; validation; pseudotypes; neutralization
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Ferrara, F.; Molesti, E.; Scott, S.; Cattoli, G.; Temperton, N. The Use of Hyperimmune Chicken Reference Sera Is Not Appropriate for the Validation of Influenza Pseudotype Neutralization Assays. Pathogens 2017, 6, 45.

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