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Pathogens 2017, 6(2), 22; doi:10.3390/pathogens6020022

Antibiotic Resistance and Virulence Phenotypes of Recent Bacterial Strains Isolated from Urinary Tract Infections in Elderly Patients with Prostatic Disease

1
Earth, Environmental and Life Sciences Section, Research Institute of the University of Bucharest (ICUB), 91–95 Independenţei Avenue, 0500088 Bucharest, Romania
2
Iancului Private Laboratory, 060101 Bucharest, Romania
3
Department of Microbiology & Immunology, Faculty of Biology, University of Bucharest, 1–3 Portocalelor Lane, Sector 6, 060101 Bucharest, Romania
4
Ştefan S. Nicolau Institute of Virology, 285 Mihai Bravu Avenue, 030304 Bucharest, Romania
5
Department of Pharmacognosy & Phytotherapy, Faculty of Pharmacy, University of Medicine and Pharmacy of Craiova, 2 Petru Rareş Street, 200349 Craiova, Romania
6
Dr. Gluck Private Practice, 060101 Bucharest, Romania
*
Authors to whom correspondence should be addressed.
Academic Editor: Alexandru Mihai Grumezescu
Received: 24 February 2017 / Revised: 18 May 2017 / Accepted: 25 May 2017 / Published: 31 May 2017
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Abstract

Acute bacterial prostatitis is one of the frequent complications of urinary tract infection (UTI). From the approximately 10% of men having prostatitis, 7% experience a bacterial prostatitis. The purpose of this study was to investigate the prevalence of uropathogens associated with UTIs in older patients with benign prostatic hyperplasia and to assess their susceptibility to commonly prescribed antibiotics as well as the relationships between microbial virulence and resistance features. Uropathogenic Escherichia coli was found to be the most frequent bacterial strain isolated from patients with benign prostatic hyperplasia, followed by Enterococcus spp., Enterobacter spp., Klebsiella spp., Proteus spp., Pseudomonas aeruginosa, and Serratia marcescens. Increased resistance rates to tetracyclines, quinolones, and sulfonamides were registered. Besides their resistance profiles, the uropathogenic isolates produced various virulence factors with possible implications in the pathogenesis process. The great majority of the uropathogenic isolates revealed a high capacity to adhere to HEp-2 cell monolayer in vitro, mostly exhibiting a localized adherence pattern. Differences in the repertoire of soluble virulence factors that can affect bacterial growth and persistence within the urinary tract were detected. The Gram-negative strains produced pore-forming toxins—such as hemolysins, lecithinases, and lipases—proteases, siderophore-like molecules resulted from the esculin hydrolysis and amylases, while Enterococcus sp. strains were positive only for caseinase and esculin hydrolase. Our study demonstrates that necessity of investigating the etiology and local resistance patterns of uropathogenic organisms, which is crucial for determining appropriate empirical antibiotic treatment in elderly patients with UTI, while establishing correlations between resistance and virulence profiles could provide valuable input about the clinical evolution and recurrence rates of UTI. View Full-Text
Keywords: urinary tract infections; antibiotic resistance; biofilm urinary tract infections; antibiotic resistance; biofilm
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MDPI and ACS Style

Delcaru, C.; Podgoreanu, P.; Alexandru, I.; Popescu, N.; Măruţescu, L.; Bleotu, C.; Mogoşanu, G.D.; Chifiriuc, M.C.; Gluck, M.; Lazăr, V. Antibiotic Resistance and Virulence Phenotypes of Recent Bacterial Strains Isolated from Urinary Tract Infections in Elderly Patients with Prostatic Disease. Pathogens 2017, 6, 22.

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