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Pathogens 2016, 5(3), 54; doi:10.3390/pathogens5030054

Genomic Recombination Leading to Decreased Virulence of Group B Streptococcus in a Mouse Model of Adult Invasive Disease

1
Public Health Ontario Laboratory, 661 University Avenue, Suite 17-100, Toronto, ON M5G 1M1, Canada
2
Laboratory of Immunology, Faculty of Veterinary Medicine, University of Montreal, 3200 Sicotte Street, Saint-Hyacinthe, QC J2S 2M2, Canada
3
McGill University and Genome Quebec Innovation Centre, 740 Dr. Penfield Avenue Rm 7104, Montreal, QC H3A 0G1, Canada
4
Department of Laboratory Medicine and Pathobiology, Faculty of Medicine, University of Toronto, 27 King’s College Circle, Toronto, ON M5S 1A1, Canada
These authors contributed equally to this work.
Present address: Department of Laboratory Medicine and Pathobiology, Faculty of Medicine, University of Toronto, 27 King’s College Circle, Toronto, ON M5S 1A1, Canada
*
Author to whom correspondence should be addressed.
Academic Editor: Michael Otto
Received: 5 July 2016 / Revised: 21 July 2016 / Accepted: 1 August 2016 / Published: 5 August 2016
View Full-Text   |   Download PDF [4155 KB, uploaded 5 August 2016]   |  

Abstract

Adult invasive disease caused by Group B Streptococcus (GBS) is increasing worldwide. Whole-genome sequencing (WGS) now permits rapid identification of recombination events, a phenomenon that occurs frequently in GBS. Using WGS, we described that strain NGBS375, a capsular serotype V GBS isolate of sequence type (ST)297, has an ST1 genomic background but has acquired approximately 300 kbp of genetic material likely from an ST17 strain. Here, we examined the virulence of this strain in an in vivo model of GBS adult invasive infection. The mosaic ST297 strain showed intermediate virulence, causing significantly less systemic infection and reduced mortality than a more virulent, serotype V ST1 isolate. Bacteremia induced by the ST297 strain was similar to that induced by a serotype III ST17 strain, which was the least virulent under the conditions tested. Yet, under normalized bacteremia levels, the in vivo intrinsic capacity to induce the production of pro-inflammatory cytokines was similar between the ST297 strain and the virulent ST1 strain. Thus, the diminished virulence of the mosaic strain may be due to reduced capacity to disseminate or multiply in blood during a systemic infection which could be mediated by regulatory factors contained in the recombined region. View Full-Text
Keywords: group B Streptococcus; Streptococcus agalactiae; recombination; invasive bacterial infection; adult infection; cytokines group B Streptococcus; Streptococcus agalactiae; recombination; invasive bacterial infection; adult infection; cytokines
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Teatero, S.; Lemire, P.; Dewar, K.; Wasserscheid, J.; Calzas, C.; Mallo, G.V.; Li, A.; Athey, T.B.; Segura, M.; Fittipaldi, N. Genomic Recombination Leading to Decreased Virulence of Group B Streptococcus in a Mouse Model of Adult Invasive Disease. Pathogens 2016, 5, 54.

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