Pathogens 2013, 2(1), 92-104; doi:10.3390/pathogens2010092
Article

Heterogeneity of the Abnormal Prion Protein (PrPSc) of the Chandler Scrapie Strain

Prion Disease Research Center, National Institute of Animal Health, Tsukuba, Ibaraki 305-0856, Japan
* Author to whom correspondence should be addressed.
Received: 9 January 2013; in revised form: 7 February 2013 / Accepted: 9 February 2013 / Published: 18 February 2013
(This article belongs to the Special Issue Prions)
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Abstract: The pathological prion protein, PrPSc, displays various sizes of aggregates. In this study, we investigated the conformation, aggregation stability and proteinase K (PK)-sensitivity of small and large PrPSc aggregates of mouse-adapted prion strains. We showed that small PrPSc aggregates, previously thought to be PK-sensitive, are resistant to PK digestion. Furthermore, we showed that small PrPSc aggregates of the Chandler scrapie strain have greater resistance to PK digestion and aggregation-denaturation than large PrPSc aggregates of this strain. We conclude that this strain consists of heterogeneous PrPSc.
Keywords: prion; Chandler; small PrPSc aggregate; conformational stability; PK sensitivity

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MDPI and ACS Style

Kasai, K.; Iwamaru, Y.; Masujin, K.; Imamura, M.; Mohri, S.; Yokoyama, T. Heterogeneity of the Abnormal Prion Protein (PrPSc) of the Chandler Scrapie Strain. Pathogens 2013, 2, 92-104.

AMA Style

Kasai K, Iwamaru Y, Masujin K, Imamura M, Mohri S, Yokoyama T. Heterogeneity of the Abnormal Prion Protein (PrPSc) of the Chandler Scrapie Strain. Pathogens. 2013; 2(1):92-104.

Chicago/Turabian Style

Kasai, Kazuo; Iwamaru, Yoshifumi; Masujin, Kentaro; Imamura, Morikazu; Mohri, Shirou; Yokoyama, Takashi. 2013. "Heterogeneity of the Abnormal Prion Protein (PrPSc) of the Chandler Scrapie Strain." Pathogens 2, no. 1: 92-104.

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