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Insects 2017, 8(2), 63; doi:10.3390/insects8020063

Isolation and Characterization of Microsatellite Loci for Cotesia plutellae (Hymenoptera: Braconidae)

1,2,3
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1,2,3
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1,4
,
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and
1,2,3,*
1
Institute of Applied Ecology and Research Centre for Biodiversity and Eco-Safety, Fujian Agriculture and Forestry University, Fuzhou 350002, China
2
Fujian-Taiwan Joint Centre for Ecological Control of Crop Pests, Fujian Agriculture and Forestry University, Fuzhou 350002, China
3
Key Laboratory of Integrated Pest Management of Fujian and Taiwan, China Ministry of Agriculture, Fuzhou 350002, China
4
Department of Botany, University of British Columbia, Vancouver, BC V6T 1Z4, Canada
*
Author to whom correspondence should be addressed.
Academic Editors: Brian T. Forschler, Kenneth Wilson, Fleur Ponton and Sheena Cotter
Received: 9 March 2017 / Revised: 21 May 2017 / Accepted: 12 June 2017 / Published: 20 June 2017
(This article belongs to the Special Issue Parasite-Insect Interactions)
View Full-Text   |   Download PDF [504 KB, uploaded 20 June 2017]   |  

Abstract

Fourteen polymorphic microsatellite loci were isolated in this transcriptome-based data analysis for Cotesia plutellae, which is an important larval parasitoid of the worldwide pest Plutella xylostella. A subsequent test was performed for a wild C. plutellae population (N = 32) from Fuzhou, Fujian, southeastern China, to verify the effectiveness of the 14 microsatellite loci in future studies on C. plutellae genetic diversity. The observed number of alleles ranged from two to six. The expected and observed heterozygosity ranged from 0.123 to 0.316 and from 0.141 to 0.281, respectively. The polymorphism information content (PIC) value ranged from 0.272 to 0.622. Potentially due to the substructure of the sampled population, three of the 14 microsatellite loci deviated from Hardy—Weinberg equilibrium (HWE). Further, loci C6, C22, and C31 could be amplified in Cocobius fulvus and Encarsia japonica, suggesting the transferability of these three polymorphic loci to other species of Hymenoptera. View Full-Text
Keywords: microsatellite loci; Hymenoptera; genetic variation; polymorphism microsatellite loci; Hymenoptera; genetic variation; polymorphism
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MDPI and ACS Style

Liu, T.; Ke, F.; You, S.; Chen, W.; He, W.; You, M. Isolation and Characterization of Microsatellite Loci for Cotesia plutellae (Hymenoptera: Braconidae). Insects 2017, 8, 63.

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