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Insects 2015, 6(4), 827-846; doi:10.3390/insects6040827

Molecular Mechanism Underlying the Entomotoxic Effect of Colocasia esculenta Tuber Agglutinin against Dysdercus cingulatus

Division of Plant Biology, Bose Institute, Centenary Campus, P1/12, CIT Scheme, VIIM, Kakurgachi, Kolkata 700054, West Bengal, India
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Author to whom correspondence should be addressed.
Academic Editor: Brian T. Forschler
Received: 13 June 2015 / Revised: 27 August 2015 / Accepted: 14 September 2015 / Published: 7 October 2015
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Abstract

Colocasia esculenta tuber agglutinin (CEA), a mannose binding lectin, exhibits insecticidal efficacy against different hemipteran pests. Dysdercus cingulatus, red cotton bug (RCB), has also shown significant susceptibility to CEA intoxication. However, the molecular basis behind such entomotoxicity of CEA has not been addressed adequately. The present study elucidates the mechanism of insecticidal efficacy of CEA against RCB. Confocal and scanning electron microscopic analyses documented CEA binding to insect midgut tissue, resulting in an alteration of perimicrovillar membrane (PMM) morphology. Internalization of CEA into insect haemolymph and ovary was documented by western blotting analyses. Ligand blot followed by mass spectrometric identification revealed the cognate binding partners of CEA as actin, ATPase and cytochrome P450. Deglycosylation and mannose inhibition assays indicated the interaction to probably be mannose mediated. Bioinformatic identification of putative glycosylation or mannosylation sites in the binding partners further supports the sugar mediated interaction. Correlating entomotoxicity of CEA with immune histological and binding assays to the insect gut contributes to a better understanding of the insecticidal potential of CEA and endorses its future biotechnological application. View Full-Text
Keywords: Colocasia esculenta tuber agglutinin; hemipteran pest; MALDI TOF/TOF; perimicrovillar membrane (PMM); entomotoxicity Colocasia esculenta tuber agglutinin; hemipteran pest; MALDI TOF/TOF; perimicrovillar membrane (PMM); entomotoxicity
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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#, A.R.; Das, S. Molecular Mechanism Underlying the Entomotoxic Effect of Colocasia esculenta Tuber Agglutinin against Dysdercus cingulatus. Insects 2015, 6, 827-846.

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