Ozone Effectiveness on Wheat Weevil Suppression: Preliminary Research
Abstract
:1. Introduction
2. Materials and Methods
2.1. Ozonator
2.2. Insects and Ozonation
2.3. Data Analysis
3. Results
3.1. Efficiency of Ozone in Wheat Weevil Suppression
3.2. The Influence of Ozone on the Walking Response and Velocity of Wheat Weevils
4. Discussion
5. Conclusions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
- Pimentel, D. World resources and food losses to pests, in ecology and management of food industry pests. In FDA Technical Bulletin, 4th ed.; Gorham, J.R., Ed.; Association of Official Analytical Chemists: Arlington, VA, USA, 1991; pp. 5–11. [Google Scholar]
- Harein, P.; Meronuck, R. Stored grain losses due to insects and molds and the importance of proper grain management. In U: Stored Product Management; Krischik, V., Cuperus, G., Galliart, D., Eds.; Oklahoma State University: Stillwater, OK, USA, 1995; pp. 29–31. [Google Scholar]
- Hansen, L.S.; Hansen, P.; Jensen, K.V. Lethal doses of ozone for control of all stages of internal and external feeders in stored products. Pest Manag. Sci. 2012, 68, 1311–1316. [Google Scholar] [CrossRef]
- Rees, D.P. Coleoptera in integrated management of insects in stored products. In Fundamentals of Stored-Product Entomology; Subramanyam, B., Hagstrum, D.W., Eds.; Marcel Dekker, Inc.: New York, NY, USA, 1996; pp. 1–39. [Google Scholar]
- Shelton, M.D.; Walter, V.R.; Brandl, D.; Mendez, V. The effects of refrigerated, controlled atmosphere storage during marine shipment on insect morality and cut-ßower vase life. HortTechnology 1996, 6, 247–250. [Google Scholar] [CrossRef]
- Johnson, J.A.; Vail, P.V.; Soderstrom, E.L.; Curtis, C.E.; Brandl, D.G.; Tebbets, J.S.; Valero, K.A. Integration of nonchemical, postharvest treatments for control of navel orangeworm (Lepidoptera: Pyralidae) and Indianmeal moth (Lepidoptera: Pyralidae) in walnuts. J. Econ. Entomol. 1998, 91, 1437–1444. [Google Scholar] [CrossRef]
- Zettler, J.L.; Halliday, W.R.; Arthur, F.H. Phosphine resistance in insects infesting stored peanuts in the southeastern United States. J. Econ. Entomol. 1989, 82, 1508–1511. [Google Scholar] [CrossRef]
- Zettler, J.L.; Cuperusi, G.W. Pesticide resistance in Tribolium castaneum (Coleoptera: Tenebrionidae) and Rhyzopertha dominica (Coleoptera: Bostrichidae) in wheat. J. Econ. Entomol. 1990, 83, 1677–1681. [Google Scholar] [CrossRef]
- Benhalima, H.; Chaudhry, M.Q.; Mills, K.A.; Price, N.R. Phosphine resistance in stored-product insects collected from various grain storage facilities in Morocco. J. Stored Prod. Res. 2004, 40, 241–249. [Google Scholar] [CrossRef]
- Erdman, H.E. Ozone toxicity during ontogeny of two species of flour beetles, Tribolium confusum and T. castaneum. Environ. Entomol. 1980, 9, 16–17. [Google Scholar] [CrossRef]
- Strait, C.A. Efficacy of Ozone to Control Insects and Fungi in Stored Grain. Master’s Thesis, Purdue University, West Lafayette, IN, USA, 1998. [Google Scholar]
- Kells, S.A.; Mason, L.J.; Maier, D.E.; Woloshuk, C.P. Efficacy and fumigation characteristics of ozone in stored maize. J. Stored Prod. Res. 2001, 37, 371–382. [Google Scholar] [CrossRef]
- Leesch, J.G. The mortality of stored-product insects following exposure to gaseous ozone at high concentrations. In Proceedings of the 8th International Working Conference on Stored-Product Protection, York, UK, 22–26 July 2002; Credland, P.F., Armitage, D.M., Bell, C.H., Cogan, P.M., Highley, E., Eds.; CAB International: Oxon, UK, 2003; pp. 827–831. [Google Scholar]
- Zhanggui, Q.; Xia, W.; Gang, D.; Xiaoping, Y.; Xuechao, H.; Deke, X.; Xingwen, L. Investigation of the use of ozone fumigation to control several species of stored grain insects. In Proceedings of the 8th International Working Conference on Stored-Product Protection, York, UK, 22–26 July 2002; Credland, P.F., Armitage, D.M., Bell, C.H., Cogan, P.M., Highley, E., Eds.; CAB International: Oxon, UK, 2003; pp. 846–851. [Google Scholar]
- Isikber, A.A.; Öztekin, S. Comparison of two stored-product insects, Ephestia kuehniella Zeller and Tribolium confusum du Val to gaseous ozone. J. Stored Prod. Res. 2009, 45, 159–164. [Google Scholar] [CrossRef]
- Tiwari, B.K.; Brennan, C.S.; Curran, T.; Gallagher, E.; Cullen, P.J.; O’Donnell, C.P. Application of ozone in grain processing. J. Cereal Sci. 2010, 51, 248–255. [Google Scholar] [CrossRef]
- Hollingsworth, R.G.; Armstrong, J.W. Potential of temperature, controlled atmospheres, and Ozone fumigation to control Thrips and Mealybugs on ornamental plants for export. J. Econ. Entomol. 2005, 98, 289–298. [Google Scholar] [CrossRef]
- Health Effects of Ozone Pollution. Available online: https://www.epa.gov/ground-level-ozone-pollution/health-effects-ozone-pollution (accessed on 14 August 2019).
- Ozone. Available online: https://www.lenntech.com/ozone.htm (accessed on 14 March 2019).
- Msayleb, N. Ozone as a Safer and Greener Alternative to Pesticides. Available online: https://theglobalscientist.com/2015/01/07/ozone-as-a-safer-and-greener-alternative-to-pesticides/ (accessed on 25 March 2019).
- Ozone. Available online: https://www.nasa.gov/ozone (accessed on 2 April 2019).
- Law, S.E.; Kiss, E.G. Instrumentation for ozone-based insect control in agriculture. In Proceedings of the ASAE Symposium on Automated Agriculture for the 21st Century, Chicago, IL, USA, 16–17 December 1991. [Google Scholar]
- Pureox Gaseous Ozone Fumigation and Sterilization. Available online: http://www.cosmedgroup.com/pureox.html (accessed on 20 March 2019).
- Legeron, J.P. Ozone disinfection of drinking water. In Handbook of Ozone Technology and Applications; Rice, R.G., Netzer, A., Eds.; Butterworth: Boston, MA, USA, 1984; pp. 99–121. [Google Scholar]
- Suffet, I.H.; Anselme, C.; Mallevialle, J. Removal of tastes and odors by ozonation. In Proceedings of the American Water Works Association Annual Conference, Seminar on Ozonation, Denver, CO, USA, 1986. [Google Scholar]
- United States Environmental Protection Agency. Available online: https://www.epa.gov/ground-level-ozone-pollution/health-effects-ozone-pollution (accessed on 2 April 2019).
- Kim, J.G.; Yousef, A.E.; Dave, S. Application of ozone to control insects, molds, and mycotoxins. In Proceedings of the International Conference on Control Atmosphere and Fumigation of Stored Products, Nicosia, Cyprus, 21–26 April 1999. [Google Scholar]
- Prudente, A.D.; King, J.M. Efficacy and safety evaluation of ozonation to degrade aflatoxin in corn. J. Food Sci. 2002, 67, 2866–2872. [Google Scholar]
- Sopher, C.D.; Graham, D.M.; Rice, R.G.; Strasser, J.H. Studies on the use of ozone in production agriculture and food processing. In Proceedings of the International Ozone Association, Pan American Group, Raleigh-Durham, NC, USA, 18–22 May 2002; pp. 1–15. [Google Scholar]
- Inan, F.; Pala, M.; Doymaz, I. Use of ozone in detoxification of aflatoxin B1 in red pepper. J. Stored Prod. Res. 2007, 43, 425–429. [Google Scholar] [CrossRef]
- White, S.D.; Murphy, P.T.; Bern, C.J.; van Leeuwen, J.H. Controlling deterioration of high-moisture maize with ozone treatment. J. Stored Prod. Res. 2010, 46, 7–12. [Google Scholar] [CrossRef]
- Dollear, F.G.; Mann, G.E.; Codifer, L.P., Jr.; Gardner, H.K., Jr.; Koltun, S.P.; Vix, H.L.E. Elimination of aflatoxins from peanut meal. Am. Oilseed Soc. 1968, 45, 862–865. [Google Scholar] [CrossRef]
- Rich, S.; Tomlinson, H. Effects of ozone on conidiophores and conidia of Alternaria solani. Phytopathology 1968, 58, 444–446. [Google Scholar]
- Maeba, H.; Takamoto, Y.; Kamimura, M.; Miura, T. Destruction and detoxification of aflatoxin with ozone. J. Food Sci. 1988, 53, 667–668. [Google Scholar] [CrossRef]
- Sousa, A.H.; Faroni, L.R.D.; Guedes, R.N.C.; T’otola, M.R.; Urruchi, W.I. Ozone as a management alternative against phosphine-resistant insect pests of stored products. J. Stored Prod. Res. 2008, 44, 379–385. [Google Scholar] [CrossRef]
- E, X.; Subramanyam, B.; Li, B. Efficacy of Ozone against Phosphine susceptible and resistant strains of four stored-product insect species. Insects 2017, 8, 42. [Google Scholar] [CrossRef]
- Bonjour, E.L.; Opit, G.P.; Hardin, J.; Jones, C.L.; Payton, M.E.; Beeby, R.L. Efficacy of ozone fumigation against the major grain pests in stored wheat. J. Econ. Entomol. 2011, 104, 308–316. [Google Scholar] [CrossRef]
- Mason, L.J.; Woloshuk, C.P.; Maier, D.E. Efficacy of ozone to control insects, molds and mycotoxin. In Proceedings of the International Conference on Control Atmosphere and Fumigation of Stored Products, Nicosia, Cipar, 21–26 April 1997; pp. 665–670. [Google Scholar]
- Takigawa, K.; Ueno, K.; Nagatomo, T.; Mitsugi, F.; Ikegami, T.; Ebihara, K.; Nakamura, N.; Hashimoto, Y.; Yamashita, Y. Experiment of Pest Control with Portable Ozone Mist Device. 2011. Available online: https://www.ispc-conference.org/ispcproc/ispc21/ID136.pdf (accessed on 31 March 2019).
- Mahroof, R.M.; Amoah, B.A.; Wrighton, J. Efficacy of Ozone against the life stages of Oryzaephilus mercator (Coleoptera: Silvanidae). J. Econ. Entomol. 2018, 111, 470–481. [Google Scholar] [CrossRef]
- McDonough, M.X.; Mason, L.J.; Woloshuk, C.P. Susceptibility of stored product insects to high concentrations of ozone at different exposure intervals. J. Stored Prod. Res. 2011, 42, 306–310. [Google Scholar] [CrossRef]
- Tiens Ozonatori. Available online: http://www.tiens.hr/produkt/tiens-fruit-vegetable-cleaner/ (accessed on 24 April 2019).
- Abbott, W.S. A method of computing the effectiveness of an insecticide. J. Econ. Entomol. 1925, 18, 265–267. [Google Scholar] [CrossRef]
- Gylling Data Management Inc. ARM 2019® GDM Software; Revision 2019.4; Gylling Data Management Inc.: Brookings, SD, USA, 2019. [Google Scholar]
- Sousa, A.H.; Faroni, L.R.A.; Silva, G.N.; Guedes, R.N.C. Ozone toxicity and walking RESPONSE of populations of Sitophilus zeamais (Coleoptera: Curculionidae). J. Econ. Entomol. 2012, 105, 2187–2195. [Google Scholar] [CrossRef] [PubMed]
Variants in the Experiment (Ozone Exposure in Minutes) | The Total Amount of Ozone Per Variant (mg) | The Applied Concentration of Ozone in Air (ppm) |
---|---|---|
10 | 25 | 0.0002 |
20 | 50 | 0.0003 |
30 | 75 | 0.0005 |
60 | 150 | 0.0010 |
120 | 300 | 0.0020 |
OzonationType | Duration of Ozonation (min) | Days after Ozonation | |||||
---|---|---|---|---|---|---|---|
1 | 2 | 3 | 7 | 10 | 15 | ||
Directly to insects | 10 | 0.0 ± 0.0 c * | 0.0 ± 0.0 c | 5.3 ± 0.4 c | 13.5 ± 0.1 c | 25.9 ± 1.5 bc | 58.9 ± 26.7 a–d |
20 | 0.0 ± 0.0 c | 0.3 ± 0.8 bc | 11.4 ± 9.4 bc | 20.9 ± 0.3 c | 36.6 ± 2.9 bc | 59.6 ± 28.8 a–d | |
30 | 0.6 ± 6.5 bc | 0.7 ± 1.3 bc | 1.9 ± 9.45 c | 6.6 ± 0.5 c | 21.5 ± 2.1 bc | 45.6 ± 6.4 bcd | |
60 | 6.1 ± 11.3 abc | 11.7 ± 0.3 ab | 12.8 ± 3.8 bc | 42.1 ± 0.2 bc | 67.5 ± 1.5 ab | 88.2 ± 16.6 abc | |
120 | 17.3 ± 8.9 a | 28.5 ± 0.9 a | 69.8 ± 8.1 a | 100.0 ± 0.0 a | 100.0 ± 0.0 a | 100.0 ± 0.0 a | |
Insects in grain | 10 | 0.0 ± 0.0 c | 2.1 ± 1.3 bc | 2.1 ± 9.9 c | 7.2 ± 0.5 c | 8.7 ± 0.7 c | 20.7 ± 14.3 cd |
20 | 0.0 ± 0.0 c | 0.7 ± 1.4 bc | 2.0 ± 9.7 c | 9.5 ± 0.2 c | 14.2 ± 1.1 c | 17.0 ± 10.3 d | |
30 | 0.0 ± 0.0 c | 1.3 ± 1.0 bc | 1.3 ± 7.7 c | 11.0 ± 0.2 c | 12.7 ± 1.2 c | 13.8 ± 9.4 d | |
60 | 2.4 ± 12.7 abc | 6.3 ± 1.6 abc | 14.9 ± 15.1 bc | 24.3 ± 0.5 c | 31.3 ± 2.7 bc | 44.2 ± 33.2 bcd | |
120 | 7.8 ± 13.4 ab | 24.2 ± 1.1 a | 41.3 ± 9.6 ab | 76.9 ± 0.2 ab | 94.5 ± 0.4 a | 97.8 ± 10.1 ab | |
HSD ** p = 0.05 | 11.5 | 14.9 | 22.8 | 38.4 | 48.0 | 50.4 |
Ozonation Type | Duration of Ozonation (min) | Days after Ozonation | |||||
---|---|---|---|---|---|---|---|
1 | 2 | 3 | 7 | 10 | 15 | ||
Directly to insects | 10 | 45.5 ± 5.1 ab * | 45.0 ± 15.8 abc | 76.8 ± 5.8 ab | 65.0 ± 24.3 a | 24.3 a ± 24.3 a | 44.0 ± 20.1 a |
20 | 64.8 ± 14.2 a | 67.8 ± 18.0 a | 84.0 ± 16.2 a | 69.5 ± 4.5 a | 4.5 a ± 4.5 a | 23.8 ± 24.9 ab | |
30 | 40.5 ± 17.2 ab | 63.0 ± 17.2 ab | 71.0 ± 11.7 ab | 48.3 ± 17.0 ab | 17.0 ab ± 17.0 ab | 16.5 ± 14.0 ab | |
60 | 59.8 ± 26.6 a | 56.8 ± 1.7 abc | 54.5 ± 18.1 abc | 51.3 ± 13.5 ab | 13.5 ab ± 13.5 ab | 17.3 ± 25.5 ab | |
120 | 0.0 ± 0.0 b | 17.5 ± 35.0 c | 12.5 ± 25.0 c | 0.0 ± 0.0 c | 0.0 c ± 0.0 c | 0.0 ± 0.0 b | |
Insects in grain | 10 | 45.0 ± 13.3 ab | 36.8 ± 10.9 abc | 43.8 ± 2.4 abc | 46.0 ± 3.8 ab | 44.5 ± 10.4 ab | 33.3 ± 6.2 ab |
20 | 35.8 ± 11.6 ab | 44.3 ± 8.4 abc | 39.0 ± 28.7 abc | 42.3 ± 13.1 ab | 43.5 ± 11.6 ab | 31.5 ± 14.6 ab | |
30 | 42.5 ± 20.2 ab | 32.0 ± 4.8 abc | 40.8 ± 13.9 abc | 48.0 ± 17.6 ab | 44.8 ± 11.5 ab | 41.3 ± 11.4 a | |
60 | 49.3 ± 16.9 a | 57.3 ± 14.8 abc | 46.8 ± 16.1 abc | 48.3 ± 15.5 ab | 42.8 ± 28.7 ab | 27.3 ± 20.0 ab | |
120 | 51.0 ± 39.7 a | 23.8 ± 28.7 bc | 30.8 ± 36.7 bc | 16.8 ± 18.0 c | 7.8 ± 9.0 b | 0.0 ± 0.0 b | |
HSD ** p = 0.05 | 46.1 | 41.8 | 0.4 | 35.2 | 44.8 | 41.2 |
Ozonation Type | Duration of Ozonation (min) | Days after Ozonation | |||||
---|---|---|---|---|---|---|---|
1 | 2 | 3 | 7 | 10 | 15 | ||
Directly to insects | 10 | 155.0 ± 5.8 ab * | 137.5 ± 9.6 ns | 9.6 ns ± 33.2 ab | 117.5 ± 71.8 a | 117.5 ± 79.3 ab | 90.0 ± 29.4 ab |
20 | 125.0 ± 19.2 abc | 115.0 ± 33.2 ns | 33.2 ns ± 56.6 abc | 77.5 ± 55.6 ab | 77.5 ± 47.9 abc | 107.5 ± 99.1 ab | |
30 | 142.5 ± 9.6 ab | 120.0 ± 21.6 ns | 21.6 ns ± 9.6 ab | 112.5 ± 17.1 a | 112.5 ± 18.3 abc | 90.0 ± 57.2 ab | |
60 | 107.5 ± 9.6 bc | 105.0 ± 23.8 ns | 23.8 ns ± 25.0 abc | 100.0 ± 43.2 ab | 100.0 ± 49.9 abc | 55.0 ± 65.6 ab | |
120 | 82.5 ± 35.0 c | 85.0 ± 20.8 ns | 20.8 ns ± 42.7 c | 0.0 ± 0.0 b | 0.0 ± 0.0 c | 0.0 ± 0.0 b | |
Insects in grain | 10 | 155.0 ± 12.9 ab | 152.5 ± 28.7 ns | 155.0 ± 12.9 a | 140.0 ± 8.2 a | 135.0 ± 23.8 ab | 127.5 ± 45.7 ab |
20 | 165.0 ± 17.3 a | 157.5 ± 17.1 ns | 160.0 ± 16.3 a | 157.5 ± 22.2 a | 172.5 ± 22.2 a | 147.5 ± 46.5 a | |
30 | 160.0 ± 14.1 ab | 145.0 ± 10.0 ns | 127.5 ± 17.0 ab | 120.0 ± 20.0 | 170.0 ± 8.2 a | 150.0 ± 24.5 a | |
60 | 140.0 ± 40.0 ab | 115.0 ± 63.5 ns | 112.5 ± 68.5 abc | 97.5 ± 63.4 ab | 102.5 ± 77.2 abc | 105.0 ± 71.4 ab | |
120 | 107.5 ± 37.8 bc | 100.0 ± 59.4 ns | 57.5 ± 40.3 bc | 70.0 ± 54.8 ab | 35.0 ± 41.2 bc | 5.0 ± 5.8 d | |
HSD ** p = 0.05 | 56.5 | 80.7 | 91.8 | 107.8 | 113.8 | 132.2 |
Days after Ozonation | Ozonation Type | LC50 | 95% Confidence Limits | Regression Line Equation |
---|---|---|---|---|
1 | Directly to insects | 701.69 | 544.58–1037.22 | Y = −1.0622 + 2.1300 X |
Insects in grain | 1032.21 | 721.94–1883.66 | Y = −0.9277 + 1.9669 X | |
2 | Directly to insects | 528.69 | 444.82–664.74 | Y = −1.0594 + 2.2251 X |
Insects in grain | 1505.63 | 993.30–2716.34 | Y = 1.2821 + 1.1700 X | |
3 | Directly to insects | 255.59 | 234.33–281.96 | Y = −1.3817 + 2.6507 X |
Insects in grain | 477.68 | 400.71–593.03 | Y = 0.5588 + 1.6577 X | |
7 | Directly to insects | 210.48 | 184.72–249.95 | Y = −1.0686 + 2.6121 X |
Insects in grain | 206.81 | 188.42–229.58 | Y = 0.1640 + 2.0885 X | |
10 | Directly to insects | 127.66 | 118.91–138.69 | Y = −1.1763 + 2.9326 X |
Insects in grain | 161.46 | 147.53–178.79 | Y = 0.0981 + 2.2200 X | |
15 | Directly to insects | 101.02 | 95.84–106.56 | Y = −2.2073 + 3.5958 X |
Insects in grain | 146.91 | 130.43–168.97 | Y = 1.6111 + 1.5638 X |
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Lemic, D.; Jembrek, D.; Bažok, R.; Pajač Živković, I. Ozone Effectiveness on Wheat Weevil Suppression: Preliminary Research. Insects 2019, 10, 357. https://doi.org/10.3390/insects10100357
Lemic D, Jembrek D, Bažok R, Pajač Živković I. Ozone Effectiveness on Wheat Weevil Suppression: Preliminary Research. Insects. 2019; 10(10):357. https://doi.org/10.3390/insects10100357
Chicago/Turabian StyleLemic, Darija, Davor Jembrek, Renata Bažok, and Ivana Pajač Živković. 2019. "Ozone Effectiveness on Wheat Weevil Suppression: Preliminary Research" Insects 10, no. 10: 357. https://doi.org/10.3390/insects10100357