Diagnostics 2014, 4(1), 13-26; doi:10.3390/diagnostics4010013

Development of a Hybrid Nanoprobe for Triple-Modality MR/SPECT/Optical Fluorescence Imaging

1,* email, 2email, 3email, 1,4,5email, 2email, 1email and 1,4email
Received: 29 January 2014; in revised form: 28 February 2014 / Accepted: 4 March 2014 / Published: 10 March 2014
(This article belongs to the Special Issue Hybrid Imaging in Medicine)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: Hybrid clinical imaging is an emerging technology, which improves disease diagnosis by combining already existing technologies. With the combination of high-resolution morphological imaging, i.e., MRI/CT, and high-sensitive molecular detection offered by SPECT/PET/Optical, physicians can detect disease progression at an early stage and design patient-specific treatments. To fully exploit the possibilities of hybrid imaging a hybrid probe compatible with each imaging technology is required. Here, we present a hybrid nanoprobe for triple modality MR/SPECT/Fluorescence imaging. Our imaging agent is comprised of superparamagnetic iron oxide nanoparticles (SPIONs), labeled with 99mTc and an Alexa fluorophore (AF), together forming 99mTc-AF-SPIONs. The agent was stable in human serum, and, after subcutaneous injection in the hind paw of Wistar rats, showed to be highly specific by accumulating in the sentinel lymph node. All three modalities clearly visualized the imaging agent. Our results show that a single imaging agent can be used for hybrid imaging. The use of a single hybrid contrast agent permits simultaneous hybrid imaging and, more conventionally, allow for single modality imaging at different time points. For example, a hybrid contrast agent enables pre-operative planning, intra-operative guidance, and post-operative evaluation with the same contrast agent.
Keywords: SPION; SPECT; optical; fluorescence imaging; magnetic resonance imaging; MR; SLN
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MDPI and ACS Style

Madru, R.; Svenmarker, P.; Ingvar, C.; Ståhlberg, F.; Engels, S.-A.; Knutsson, L.; Strand, S.-E. Development of a Hybrid Nanoprobe for Triple-Modality MR/SPECT/Optical Fluorescence Imaging. Diagnostics 2014, 4, 13-26.

AMA Style

Madru R, Svenmarker P, Ingvar C, Ståhlberg F, Engels S-A, Knutsson L, Strand S-E. Development of a Hybrid Nanoprobe for Triple-Modality MR/SPECT/Optical Fluorescence Imaging. Diagnostics. 2014; 4(1):13-26.

Chicago/Turabian Style

Madru, Renata; Svenmarker, Pontus; Ingvar, Christian; Ståhlberg, Freddy; Engels, Stefan-Andersson; Knutsson, Linda; Strand, Sven-Erik. 2014. "Development of a Hybrid Nanoprobe for Triple-Modality MR/SPECT/Optical Fluorescence Imaging." Diagnostics 4, no. 1: 13-26.

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