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Diagnostics 2014, 4(1), 1-11; doi:10.3390/diagnostics4010001
Article

An Improved Neutral a-Glucosidase Assay for Assessment of Epididymal Function—Validation and Comparison to the WHO Method

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Received: 6 November 2013 / Revised: 19 December 2013 / Accepted: 6 January 2014 / Published: 9 January 2014
(This article belongs to the collection Feature Papers)
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Abstract

Neutral a-glucosidase (NAG) activity in human seminal plasma is an important indicator for epididymis functionality. In the present study, the classic World Health Organization (WHO) method has been adapted to enhance assay robustness. Changes include modified enzyme reaction buffer composition and usage of an alternative enzyme inhibitor for background correction (glucose instead of castanospermine). Both methods have been tested in parallel on 144 semen samples, obtained from 94 patients/donors and 50 vasectomized men (negative control), respectively. Passing-Bablok regression analysis demonstrated equal assay performance. In terms of assay validation, analytical specificity, detection limit, measuring range, precision, and cut-off values have been calculated. These data confirm that the adapted method is a reliable, improved tool for NAG analysis in human semen.
Keywords: epididymis; neutral a-glucosidase; method optimization; assay validation epididymis; neutral a-glucosidase; method optimization; assay validation
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Eertmans, F.; Bogaert, V.; Van Poecke, T.; Puype, B. An Improved Neutral a-Glucosidase Assay for Assessment of Epididymal Function—Validation and Comparison to the WHO Method. Diagnostics 2014, 4, 1-11.

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