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Diagnostics 2014, 4(1), 1-11; doi:10.3390/diagnostics4010001

An Improved Neutral a-Glucosidase Assay for Assessment of Epididymal Function—Validation and Comparison to the WHO Method

Department of Research and Development, FertiPro NV, Industriepark Noord 32, 8730 Beernem, WVL, Belgium
Laboratory of Andrology, Ghent University Hospital, De Pintelaan 185, 9000 Ghent, OVL, Belgium
Author to whom correspondence should be addressed.
Received: 6 November 2013 / Revised: 19 December 2013 / Accepted: 6 January 2014 / Published: 9 January 2014
(This article belongs to the Collection Feature Papers)
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Neutral a-glucosidase (NAG) activity in human seminal plasma is an important indicator for epididymis functionality. In the present study, the classic World Health Organization (WHO) method has been adapted to enhance assay robustness. Changes include modified enzyme reaction buffer composition and usage of an alternative enzyme inhibitor for background correction (glucose instead of castanospermine). Both methods have been tested in parallel on 144 semen samples, obtained from 94 patients/donors and 50 vasectomized men (negative control), respectively. Passing-Bablok regression analysis demonstrated equal assay performance. In terms of assay validation, analytical specificity, detection limit, measuring range, precision, and cut-off values have been calculated. These data confirm that the adapted method is a reliable, improved tool for NAG analysis in human semen.
Keywords: epididymis; neutral a-glucosidase; method optimization; assay validation epididymis; neutral a-glucosidase; method optimization; assay validation

This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Eertmans, F.; Bogaert, V.; Van Poecke, T.; Puype, B. An Improved Neutral a-Glucosidase Assay for Assessment of Epididymal Function—Validation and Comparison to the WHO Method. Diagnostics 2014, 4, 1-11.

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