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TD-GC-MS Investigation of the VOCs Released from Blood Plasma of Dogs with Cancer
Graduate School of Environmental Engineering, the University of Kitakyushu, 1-1 Hibikino, Wakamatsu-ku, Kitakyushu 808-0135, Japan
Shinkou Seiki Co. Ltd., 1-18-3, Maidashi, Higashi-ku, Fukuoka 812-8581, Japan
Department of Veterinary Pathology, School of Veterinary Medicine, Rakuno Gakuen University, 582, Midorimachi, Bunkyodai, Ebetsu 069-8501, Japan
* Author to whom correspondence should be addressed.
Received: 8 December 2012; in revised form: 31 December 2012 / Accepted: 15 January 2013 / Published: 16 January 2013
Abstract: An analytical TD-GC-MS method was developed and used for the assessment of volatile organic compounds (VOCs) released from the blood plasma of dogs with/without cancer. VOCs released from 40 samples of diseased blood and 10 control samples were compared in order to examine the difference between both sample groups that were showing qualitatively similar results independent from the disease’s presence. However, mild disturbances in the spectra of dogs with cancer in comparison with the control group were observed, and six peaks (tentatively identified by comparison with mass spectral library as hexanal, octanal, toluene, 2-butanone, 1-octen-3-ol and pyrrole) revealed statistically significant differences between both sample groups, thereby suggesting that these compounds are potential biomarkers that can be used for cancer diagnosis based on the blood plasma TD-GC-MS analysis. Statistical comparison with the application of principal component analysis (PCA) provided accurate discrimination between the cancer and control groups, thus demonstrating stronger biochemical perturbations in blood plasma when cancer is present.
Keywords: dog blood plasma; TD-GC-MS; volatile organic compounds; cancer markers
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Selyanchyn, R.; Nozoe, T.; Matsui, H.; Kadosawa, T.; Lee, S.-W. TD-GC-MS Investigation of the VOCs Released from Blood Plasma of Dogs with Cancer. Diagnostics 2013, 3, 68-83.
Selyanchyn R, Nozoe T, Matsui H, Kadosawa T, Lee S-W. TD-GC-MS Investigation of the VOCs Released from Blood Plasma of Dogs with Cancer. Diagnostics. 2013; 3(1):68-83.
Selyanchyn, Roman; Nozoe, Takuma; Matsui, Hidetaka; Kadosawa, Tsuyoshi; Lee, Seung-Woo. 2013. "TD-GC-MS Investigation of the VOCs Released from Blood Plasma of Dogs with Cancer." Diagnostics 3, no. 1: 68-83.