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A Disposable Microfluidic Virus Concentration Device Based on Evaporation and Interfacial Tension
Department of Biomedical Engineering, Boston University, 44 Cummington Mall, Boston, MA 02215, USA
Division of Infectious Diseases, Beth Israel Deaconess Medical Center and Department of Lab Medicine, Boston Children's Hospital, 300 Longwood Ave, Boston, MA 02115, USA
* Author to whom correspondence should be addressed.
Received: 18 December 2012; in revised form: 12 February 2013 / Accepted: 20 February 2013 / Published: 28 February 2013
Abstract: We report a disposable and highly effective polymeric microfluidic viral sample concentration device capable of increasing the concentration of virus in a human nasopharyngeal specimen more than one order of magnitude in less than 30 min without the use of a centrifuge. The device is fabricated using 3D maskless xurography method using commercially available polymeric materials, which require no cleanroom operations. The disposable components can be fabricated and assembled in five minutes. The device can concentrate a few milliliters (mL) of influenza virus in solution from tissue culture or clinical nasopharyngeal swab specimens, via reduction of the fluid volume, to tens of microliters (mL). The performance of the device was evaluated by nucleic acid extraction from the concentrated samples, followed by a real-time quantitative polymerase chain reaction (qRT-PCR). The viral RNA concentration in each sample was increased on average over 10-fold for both cultured and patient specimens compared to the starting samples, with recovery efficiencies above 60% for all input concentrations. Highly concentrated samples in small fluid volumes can increase the downstream process speed of on-chip nucleic acid extraction, and result in improvements in the sensitivity of many diagnostic platforms that interrogate small sample volumes.
Keywords: microfluidic; sample preparation; virus; point-of-care diagnostics; influenza; PCR
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MDPI and ACS Style
Zhang, J.Y.; Mahalanabis, M.; Liu, L.; Chang, J.; Pollock, N.R.; Klapperich, C.M. A Disposable Microfluidic Virus Concentration Device Based on Evaporation and Interfacial Tension. Diagnostics 2013, 3, 155-169.
Zhang JY, Mahalanabis M, Liu L, Chang J, Pollock NR, Klapperich CM. A Disposable Microfluidic Virus Concentration Device Based on Evaporation and Interfacial Tension. Diagnostics. 2013; 3(1):155-169.
Zhang, Jane Y.; Mahalanabis, Madhumita; Liu, Lena; Chang, Jessie; Pollock, Nira R.; Klapperich, Catherine M. 2013. "A Disposable Microfluidic Virus Concentration Device Based on Evaporation and Interfacial Tension." Diagnostics 3, no. 1: 155-169.