Next Article in Journal
The Hypothesis that the Genetic Code Originated in Coupled Synthesis of Proteins and the Evolutionary Predecessors of Nucleic Acids in Primitive Cells
Next Article in Special Issue
From Vesicles to Protocells: The Roles of Amphiphilic Molecules
Previous Article in Journal / Special Issue
Protocells: At the Interface of Life and Non-Life
Article Menu

Export Article

Open AccessShort Note
Life 2015, 5(1), 459-466; doi:10.3390/life5010459

Does DNA Exert an Active Role in Generating Cell-Sized Spheres in an Aqueous Solution with a Crowding Binary Polymer?

1
Graduate School of Engineering, Mie University, Mie, 514-8507, Japan
2
Faculty of Life and Medical Sciences, Doshisha University, Kyotanabe, 610-0394, Japan
*
Author to whom correspondence should be addressed.
Academic Editors: Fabio Mavelli and Pasquale Stano
Received: 5 November 2014 / Accepted: 2 February 2015 / Published: 9 February 2015
(This article belongs to the Special Issue Protocells - Designs for Life)
View Full-Text   |   Download PDF [768 KB, uploaded 9 February 2015]   |  

Abstract

We report the spontaneous generation of a cell-like morphology in an environment crowded with the polymers dextran and polyethylene glycol (PEG) in the presence of DNA. DNA molecules were selectively located in the interior of dextran-rich micro-droplets, when the composition of an aqueous two-phase system (ATPS) was near the critical condition of phase-segregation. The resulting micro-droplets could be controlled by the use of optical tweezers. As an example of laser manipulation, the dynamic fusion of two droplets is reported, which resembles the process of cell division in time-reverse. A hypothetical scenario for the emergence of a primitive cell with DNA is briefly discussed. View Full-Text
Keywords: DNA; dextran; polyethylene glycol (PEG); crowding; aqueous two-phase system (ATPS); optical tweezers; microcompartment; artificial cell model DNA; dextran; polyethylene glycol (PEG); crowding; aqueous two-phase system (ATPS); optical tweezers; microcompartment; artificial cell model
Figures

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Review Report

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Tsumoto, K.; Arai, M.; Nakatani, N.; Watanabe, S.N.; Yoshikawa, K. Does DNA Exert an Active Role in Generating Cell-Sized Spheres in an Aqueous Solution with a Crowding Binary Polymer? Life 2015, 5, 459-466.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Life EISSN 2075-1729 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top