Portrait of a Geothermal Spring, Hunter’s Hot Springs, Oregon

Abstract

Although alkaline Hunter’s Hot Springs in southeastern Oregon has been studied extensively for over 40 years, most of these studies and the subsequent publications were before the advent of molecular methods. However, there are many field observations and laboratory experiments that reveal the major aspects of the phototrophic species composition within various physical and chemical gradients of these springs. Relatively constant temperature boundaries demark the upper boundary of the unicellular cyanobacterium, Synechococcus at 73–74 °C (the world-wide upper limit for photosynthesis), and 68–70 °C the upper limit for Chloroflexus. The upper limit for the cover of the filamentous cyanobacterium, Geitlerinema (Oscillatoria) is at 54–55 °C, and the in situ lower limit at 47–48 °C for all three of these phototrophs due to the upper temperature limit for the grazing ostracod, Thermopsis. The in situ upper limit for the cyanobacteria Pleurocapsa and Calothrix is at ~47–48 °C, which are more grazer-resistant and grazer dependent. All of these demarcations are easily visible in the field. In addition, there is a biosulfide production in some sections of the springs that have a large impact on the microbiology. Most of the temperature and chemical limits have been explained by field and laboratory experiments.

1. Introduction

Hunter’s Hot Springs, alkaline springs, with several sources in 16 ha (42.44088 °N, 120.36907 °W) are located 3.2 km north of Lakeview, Oregon (in the Goose Lake Valley of the Great Basin). They were discovered by westerners in 1832 by trappers of the Hudson’s Bay Company, and are now officially (United States Geological Survey) known as Geyser Hunter’s Hot Springs. They are privately owned as a commercial property, and, at present, the water level varies because of geothermal drilling operations. This operation and other alterations by recent human activity occurred after the studies reported here. There was a man-made geyser (“Old Perpetual”) that erupted every 40–120 s to 15–18 m at 93 °C until a few years ago. Several sources at Hunter’s Hot Springs issued at temperatures of ~93 °C, which at the elevation of 1448 m is less than 2 °C below the boiling point of pure water (94.8 °C). The heat, in general, is a result of indirect contact with the magma as well as by the friction created during shearing of the current faulting of the tilted fault block of the Abert Rim. The shear zone provides conduit for superheated water to escape upward from considerable depths through the scoriaceous basalts of the subsurface lava flows. The substrate into which the springs emerge is the lakebed of a pluvial (Pleistocene) lake, the remnant of which still exists as Goose Lake in a graben about 10+ km to the south of Lakeview. The soft and unconsolidated sediment of the springs consists of a large deposit of empty lucustrine diatom frustules that can easily be mistaken microscopically for the presence of a viable diatom population which only exists below about 35 °C.

Several of the outlet streams continue to run to ambient temperature; others are pools with a temperature gradient from center to edge. There are several similar alkaline springs in the eastern Oregon region with somewhat similar chemistries and seemingly similar microbiota (e.g., Barry’s Hot Springs, South Harney Hot Springs; Mickey Hot Springs, Borax Lake Hot Springs, Alvord Hot Springs, and others to the east, and southeast in Oregon, northern California and Nevada [1].

The macro-chemistry of Hunter’s Hot Springs has been measured a few times. There are no visible precipitates except for thin siliceous deposits on hard materials that had fallen in the springs. The major inorganic solutes are sodium (~8.6 mM), chloride (~3.2 mM), silicate (~1.2 mM), bicarbonate (~1.7 mM), and sulfate (~2.8 mM) with a specific conductance of ~1.1 mS). The source pH varies between 8.2 and 8.3. This chemistry is characteristic of the other hot springs of southeastern Oregon [1,2]. In Hunter’s the primary sulfate level was unusually high.

Temperature and chemical gradients enabled the explicit investigation of taxon composition and environmental variation in hot springs at selected points in the gradients and determine the visually sharp boundaries among different phototrophs. The primary aim of this review is to establish the justification for the well-defined upper and lower limits of the macro-constituents of these springs. These are relatively simple microbial ecosystems (compared with the taxon composition of most soils), thereby enabling detailed interrogation of the interactions between organisms and the environment. Here, I will summarize what is known about the visually apparent diversity of phototrophic bacteria (primarily cyanobacteria) that are the primary producers in this ecosystem.in what was once a relatively undisturbed hot spring in southeastern Oregon.

This paper builds on about 40 years of periodic research by R.W. Castenholz, his students and colleagues, which has resulted in over twenty publications and made Hunter’s Hot Springs, one of the best-studied hot spring systems in the world outside of Yellowstone National Park. However, most of these studies were made prior to the use of new techniques involving gene sequencing.

2. The Synechococcus/Chloroflexus Zone

The 93 °C water of some sources and water flowing downstream above 74 °C at Hunter’s presumably harbored non-photosynthetic Bacteria and Archaea, but these have not been identified. These clear water stream outpourings were generally not more than 1–3 cm in depth, although thermal pools were sometimes over a meter deep at the central source. At a mean temperature of 73–74 °C, a green edge of a biofilm or cover of Synechococcus sp. appeared and continued to about 55 °C, although below about 68–70 °C, a somewhat thicker mat with members of the Chloroflexi (e.g., Chloroflexus sp.) developed below, with the Synechococcus biofilm on top (Figure 1 and Figure 2). The green cover consisted of one or more species or strains of unicellular, rod-shaped, photoautotrophic Synechococcus to 55 °C (Figure 3). With decreases in the temperature gradient at least four thermotypes with identical morphology occurred [3,4]. This was confirmed by a later study that again recognized four or more thermotypes, each isolate with a distinctive growth rate measured over its complete temperature range [4,5]. These results, placed in phylogenetic context indicated that the highest temperature strain (University of Oregon, Culture Collection of Microorganisms from Extreme Environments strain No. 5245; OH 28) capable of growth to >70 °C, was ancestrally derived from lower temperature strains [5]. Additional information on the evolution of thermal stable Ribulose Bisphosphate Carboxylase/Oxygenase of the highest temperature and lower temperature strains has been provided [6,7]. Lower temperature strains grew with higher maximum growth rates than the high temperature type. In short, the highest temperature strain traded off a lower growth rate maximum and inability to grow at lower temperatures (<55 °C) with a higher temperature range. A similar and probably identical high temperature strain isolated from Hunter’s was characterized earlier by Meeks and Castenholz [8,9,10], who showed that this strain was an obligate thermophile with no net growth below about 55 °C. All of the Synechococcus strains, in field or culture, contain c-phycocyanin, allophycocyanin and chlorophyll a as the light-harvesting pigments.

The orange undermat of Chloroflexus, was embedded in a “gelatinous” matrix, which was probably created as an extracellular polymeric substance of Chloroflexus and other microorganisms (Figure 3). It has been shown that in cyanobacterial mats essentially all of the radiation in the visible range (~400–700 nm) is absorbed by the cyanobacterial top layer of ~1–2 mm. It was shown in other studies that near infra-red radiation penetrated the top cover of Synechococcus, although at low intensities, but at sufficient levels to allow the Chloroflexus to operate as a photoheterotroph, using bacteriochlorophyll Bchl c (740 nm maximum) and Bchla (802 and 865 nm) as major radiation-harvesting pigments [11]. The top portion of the under layer of Chloroflexi presumably obtains sufficient organic substrates produced by the overlying photoautotroph (e.g., glycolate, lipids, polyglucose with subsequent dark fermentations to acetate and propionate [4]. All of these products of Synechoccocus are available for photoheterotrophic or heterotrophic growth of Chloroflexi. Micro-stratification within the Synechococcus top layer in Yellowstone demonstrated a vertical differentiation into high light and lower light-adapted ecotypes [4,12]. Other in vivo studies of Synechococcus populations in Yellowstone hot springs (e.g., Mushroom and Octopus springs) included a demonstration of diel nitrogen-fixation and other more obvious metabolic processes that included night-time fermentation [13].

Figure 1. Hunter’s Springs from over ~93 °C at source (gray) to the green biofilm of Synechococcus (73–74 °C) that ends at 54–55 °C with the dark brown cover of Geitlerinema (Oscillatoria) cf. terebriformis, some of which has contracted to expose a salmon-colored undermat of Chloroflexus that had been hidden by the top-mat of the cyanobacterium, G. terebriformis.

Figure 1. Hunter’s Springs from over ~93 °C at source (gray) to the green biofilm of Synechococcus (73–74 °C) that ends at 54–55 °C with the dark brown cover of Geitlerinema (Oscillatoria) cf. terebriformis, some of which has contracted to expose a salmon-colored undermat of Chloroflexus that had been hidden by the top-mat of the cyanobacterium, G. terebriformis.

Figure 2. Hunter’s top mat of Synechococcus (green) with under-mat of Chloroflexus (yellow-orange) exposed in a thermal pool at 61–64 °C by blowing off the Synechococcus with a stream of water ejected from a syringe.

Figure 2. Hunter’s top mat of Synechococcus (green) with under-mat of Chloroflexus (yellow-orange) exposed in a thermal pool at 61–64 °C by blowing off the Synechococcus with a stream of water ejected from a syringe.

Figure 3. Photomicrograph of co-culture of Synechococcus (rod-like cells, 1.2 µm wide), some dividing, and Chloroflexus aurantiacus trichomes (~0.5 µm wide) in D medium at 45 °C.

Figure 3. Photomicrograph of co-culture of Synechococcus (rod-like cells, 1.2 µm wide), some dividing, and Chloroflexus aurantiacus trichomes (~0.5 µm wide) in D medium at 45 °C.

3. The Geitlerinema/Chloroflexus Zone

Although one the fastest growing isolates of Synechococcus (e.g., CCMEE strain No. 5241; OH 20) had its temperature for most rapid growth at about 45 °C [3,5], or strain 53 [3]. This temperature zone of the Synechococcus in the field did not reveal itself very often because of a dense cover of another cyanobacterium, Geitlerinema (Oscillatoria) cf. terebriformis (Figure 4). This motile oscillatorian formed a dark mat of intertwining filaments covering Synechococcus and all other phototrophs at 54–55 °C and below during most solar conditions (Figure 1 and Figure 5). This filamentous, motile cyanobacterium became the predominant phototroph up to about 54–55 °C (the highest limit and also nearly the maximum growth rate) [14]. This phenotype and 16S rDNA genotype is known from isolates from other hot springs in the eastern Oregon area, and even as distant as Saudi Arabia [4]. C-phycoerythrin is the primary light-harvesting pigment (545 nm maximum) giving the mat its dark color, although phycocyanin and allophycocyanin are present, as in almost all cyanobacteria. This cyanobacterial mat under low light was dense and continuous due to a mass of intertwining trichomes, and as a complete mat, and under low light (morning or afternoon in summer) absorbed up to 99% of visible radiation (Figure 5) and [15]. Normally, in response to high light intensity (e.g., after 9–10 am in summer and up to >1500 µE m⁻² s⁻¹ or 700 Wm⁻²) the motile trichomes of G. terebriformis responded in two ways. If the G. terebriformis mat rested on top of a rather hard “gelatinous” substrate, the main response was a mass clumping of trichomes into dense “balls” or “fascicles” (Figure 6). The rapidity of this response is temperature and light intensity dependent, e.g., [16,17,18]. Before 1967 the ecological advantage of this response was not obvious. It is now realized that the escape from high light inhibition occurs with this “clumping” phenomenon, since there is a continuous gliding motility of trichomes in contact with each other within the clump or fascicle so that no single one is exposed to high light for more that a few minutes or seconds. Since gliding motility requires a trichome rotation, the “tail-end” of a trichome is usually entangled with other trichomes, a compulsory contraction occurs. In culture, a dense, uniform suspension of trichomes in liquid medium spread in a 4.5 cm diameter glass petri plate contracts into a dense population of only 1 cm diameter in 130 s at 47 °C under ~40 Wm⁻² irradiance, from coolwhite lamps, ~6% of highest outdoor intensity [16,17,18]. The rate of re-colonization of G. terebriformis on a denuded substrate in the field was ~1 cm hr⁻¹ from the edge of an intact mat [16,17]. G. terebriformis trichomes can glide forward at ~0.5 mm⁻¹ min under ideal temperature and light intensity [19].

Figure 4. Geitlerinema terebriformis in culture (trichomes ~5 µm wide) in D medium at 45 °C.

Figure 4. Geitlerinema terebriformis in culture (trichomes ~5 µm wide) in D medium at 45 °C.

Figure 5. Geitlerinema terebriformis “mat” on right, with edge at ~55 °C, “riding over” Synechococcus topmat during low light period. The length of the view is ~6 cm (Figure 3 in [16]).

Figure 5. Geitlerinema terebriformis “mat” on right, with edge at ~55 °C, “riding over” Synechococcus topmat during low light period. The length of the view is ~6 cm (Figure 3 in [16]).

Figure 6. Geitlerinema terebriformis in contracted fascicles over Synechococcus topmat as a result of high, mid-day, summer light intensity. Temperature ~50 °C.

Figure 6. Geitlerinema terebriformis in contracted fascicles over Synechococcus topmat as a result of high, mid-day, summer light intensity. Temperature ~50 °C.

Castenholz [15] showed that at <55 °C in this and similar hot springs (e.g., Kah-nee-ta Hot Springs, Oregon), when the substrate was soft, G. terebriformis trichomes had a vertical downward “escape” movement to 1–2 mm below the substrate under high light intensity in addition to the clumping response [15,16,18] (Figure 6 and Figure 7). In addition to the consolidation of the G. terebriformis mat in response to increasing light intensity, the mat edge showed a phobic response to a temperature above 54–55 °C, i.e., upper temperature limit for growth when the stream flow changed [15]. The convergence and retreat of the mat with increasing light intensity or the advance of higher temperature also exposed, in many cases, an under-mat of orange or pink-colored Chloroflexus (Figure 8). This under-mat of Chloroflexus was visible as well at higher temperatures, below the Synechococcus top cover below about 68 °C (Figure 1 and Figure 2). G. terebriformis did not occur in at least one of the springs at Hunter’s (i.e., “Fenceline” an unofficial name) [20]. No macro-chemical differences were shown for this spring, and the basis of this difference was never established.

In all other spring streams and pools at Hunter’s, G. terebriformis was present below about 55–54 °C, completely covering the lower temperature mat of Synechococcus, at various times of day, and this often resulted in an nearly absent or discolored cover of Synechococcus, a result of the nearly complete light shield by G. terebriformis.

Figure 7. (a) Geitlerinema terebriformis “mat” in shade and 45 °C over sediment at 9:25 am, 27 July (Kahneeta Hot Springs) (Figure 4A in [15]); (b) Same “mat” at 10:00 (full sun in summer) with G. terebriformis largely absent due to downward vertical migration. Lower mat termination at meeting with cold water of river (Figure 4C in [15]).

Figure 7. (a) Geitlerinema terebriformis “mat” in shade and 45 °C over sediment at 9:25 am, 27 July (Kahneeta Hot Springs) (Figure 4A in [15]); (b) Same “mat” at 10:00 (full sun in summer) with G. terebriformis largely absent due to downward vertical migration. Lower mat termination at meeting with cold water of river (Figure 4C in [15]).

Figure 8. Geitlerinema terebriformis mat contracted and retreating under high midday light intensity at 49–53 °C, revealing Chloroflexus undermat, originally covered by the motile G. terebriformis mat during period of low light (Figure 7 in [15]).

Figure 8. Geitlerinema terebriformis mat contracted and retreating under high midday light intensity at 49–53 °C, revealing Chloroflexus undermat, originally covered by the motile G. terebriformis mat during period of low light (Figure 7 in [15]).

4. The Ostracod/Pleurocapsa/Calothrix Zone

Although G. terebriformis was quite capable of substantial growth below 47 °C [21], the dark phycoerythrin-containing mat was abruptly attenuated at about 47–48 °C, the upper temperature limit for the herbivorous, thermophilic ostracod (Thermopsis thermophile; formerly Potamocypris sp.) that was active and reproductively competent up to 47–48 °C [20,22,23]. T. thermophile and other species of ostracods have never been found in geothermal springs in Yellowstone where ephydrid flies and their larvae comprise the major grazers, but at lower temperatures (e.g., 43–44 °C). This micro-crustacean was very efficient in consuming G. terebriformis that appeared to be its “favorite food” in these springs. In laboratory feeding experiments they eliminated G. terebriformis very quickly with natural-populations [20,23] (Figure 9). However, the elimination of G. terebriformis below ~47 °C in springs and pools containing the ostracod, allowed and promoted the slow establishment of a nearly grazer-resistant population of a tough, leathery mat of nodules composed of Pleurocapsa sp. and Calothrix sp. Both contained the UV-shading pigment, scytonemin [24] (Figure 10 and Figure 11). In a few newly established springs at Hunter’s, no ostracods were present, in which case, the G. terebriformis mat extended to temperatures below about 30 °C, although it tapered out visibly with a lower suboptimal temperature below about 35 °C (Figure 12). Slow growth was demonstrated in the laboratory at temperatures as low as 28 °C [25]. The ostracod populations that were able to decimate the G. terebriformis cover and also ranged over the leathery nodules of Pleurocapsa/Calothrix averaging about 100 animals per cm² in the 40–45 °C range in one stream (Figure 11), but often reached densities of over 2000 per cm² when higher temperature water eddies circled and corralled the animals [20]. The grazer-resistant, grazer-dependent population of Pleurocapsa and Calothrix often consisted of a basal crust of Pleurocapsa that consisted of a tight aggregation of dark-colored, thick-walled cells with embedded upright-growing filaments of tapered Calothrix with basal heterocysts (Figure 13). It is almost certain that these heterocyst-containing filaments are capable of N₂-fixation, and it is quite likely that the Pleurocapsa component was benefited by this process. It was observed that the upright Calothrix filaments grow as a “lawn”, extending above the crust of Pleurocapsa, but were usually truncated by the grazing of the ostracods, only to be extended again by later growth of the Calothrix filaments [26]. In this consortium the ostracods grazed on the extended tips of Calothrix (Figure 13). Most dividing cells of Calothrix are near the base of the filaments, close to the site of N₂-fixation (i.e., the heterocysts) therefore allowing continuous growth from below. The Calothrix never revealed colorless, terminal hairs, but it is unknown whether this was a result of high phosphate concentrations, which would inhibit the growth of these hairs, or a result of the effective grazing by the ostracods (Figure 13). This community occurred downstream (<~47 °C) in the chemical gradient where combined nitrogen is diminished due to the upstream use by mainly non-nitrogen-fixing cyanobacteria [20]. Recent findings have shown that some strains of Synechococcus in Yellowstone springs fix nitrogen, but only in late afternoon and early morning [4,13]. The existence or impact of this ability has not been investigated in Hunter’s springs. It was demonstrated in culture that the effect of grazing was considerably more effective and rapid in mono-cultures of Calothrix grown on agar nutrient discs, than on Pleurocapsa on agar discs, both immersed in springs at the appropriate temperature. This was expected, since the latter forms the basal, most resistant “leathery” crust in nature (Figure 14). In one spring (“Fenceline”) where the G. terebriformis cover was absent, the ostracods fed and truncated the Synechococcus/Chloroflexus mat at about 47 °C, the normal upper temperature limit for the animals [20]. However, these animals made feeding, swimming sorties to higher temperatures, but became comatose and closed their carapaces in a short time with increasing temperature. For example, forays to over 50 °C initiated this state often in 10–20 min [20,23]. Nevertheless, the stream current (from high to low temperature) carried comatose ostracods down to favorable temperatures (i.e., below 47 °C) where swimming recovery generally occurred. In these eastern Oregon springs no other native grazers, besides ostracods, occurred, although an occasional tiger beetle was observed feeding on the ostracods, and an introduced population of “guppies” (Poecilia reticulata) thrived in some spring streams below 38 °C., and the bellies of these, upon examination, were full of ingested ostracods.

Figure 9. Synechococcus cover (>55 °C) with Geitlerinema terebriformis cover beginning at 55 °C and ending at about 48 °C due to grazing by the ostracod, Thermopsis thermophile (small white dots). (Figure 19.5 in [27]).

Figure 9. Synechococcus cover (>55 °C) with Geitlerinema terebriformis cover beginning at 55 °C and ending at about 48 °C due to grazing by the ostracod, Thermopsis thermophile (small white dots). (Figure 19.5 in [27]).

Figure 10. Geitlerinema terebriformis cover in Hunter’s stream that has been terminated at ~48 °C by ostracod grazing, revealing dark patches of Pleurocapsa/Calothrix in ostracod zone below 47–48 °C.

Figure 10. Geitlerinema terebriformis cover in Hunter’s stream that has been terminated at ~48 °C by ostracod grazing, revealing dark patches of Pleurocapsa/Calothrix in ostracod zone below 47–48 °C.

Figure 11. Pleurocapsa, leathery nodules with population of ostracods (white dots) over the area (horizontal length ~12 mm) in a Hunter’s stream at ~45 °C.

Figure 11. Pleurocapsa, leathery nodules with population of ostracods (white dots) over the area (horizontal length ~12 mm) in a Hunter’s stream at ~45 °C.

Figure 12. Newly established Synechococcus/Geitlerinema terebriformis population on soft gray substrate after 30+ days, but without the natural introduction of ostracods. In this case, the G. terebriformis cover gradually tapered out below about 35 °C much lower than 47–48 °C that would have been the temperature of truncation if ostracods were present.

Figure 12. Newly established Synechococcus/Geitlerinema terebriformis population on soft gray substrate after 30+ days, but without the natural introduction of ostracods. In this case, the G. terebriformis cover gradually tapered out below about 35 °C much lower than 47–48 °C that would have been the temperature of truncation if ostracods were present.

Figure 13. Calothrix (tapered filaments) extended from base of aggregated cells of Pleurocapsa, (non-filamentous); from piece of nodule from ~45 °C, scraped off hard substrate. Bar = 5 µm.

Figure 13. Calothrix (tapered filaments) extended from base of aggregated cells of Pleurocapsa, (non-filamentous); from piece of nodule from ~45 °C, scraped off hard substrate. Bar = 5 µm.

Figure 14. Agar nutrient slab on which Pleurocapsa had been grown uniformly in lab (in petri dish) and then placed in “Fenceline” Spring at Hunter’s at about 45 °C and allowed to be grazed upon by a natural ostracod population shown grazing here (small dark specks), after about 12 h with about 800–1000 animals cm⁻², see [20].

Figure 14. Agar nutrient slab on which Pleurocapsa had been grown uniformly in lab (in petri dish) and then placed in “Fenceline” Spring at Hunter’s at about 45 °C and allowed to be grazed upon by a natural ostracod population shown grazing here (small dark specks), after about 12 h with about 800–1000 animals cm⁻², see [20].

5. Sulfate Reducing Zone: Thermochromatium/Beggiatoa/Geitlerinema

In some slow-flowing streams and warm pools (35–40 °C) in the southern part of the Hunter’s cluster of springs, sulfide concentrations (mainly HS⁻ at pH ~8.0–8.2) have been measured at 0.5 mM at the mat surface at night and up to 0.8 mM at 2–3 mm depth even in the daylight [18]. It was obvious that the high sulfide was a result of biogenic sulfate reduction, since soluble sulfide was absent in the inorganic source waters, and H₂S arose from the mat at night with a strong odor [18]. In these pools and slow streams, at temperatures of ~50 °C and lower, a conspicuous under layer of purple sulfur bacteria (i.e., Thermochomatium cf. tepidum) appeared at the top of the mat in darkness (Figure 15). Often, these flagellated cells swarmed at night (en masse) into the somewhat toxic water above, as pink clouds with cells containing “endocellular” grains of elemental sulfur (Figure 16). Although anoxygenic photosynthesis of the purple sulfur bacteria uses soluble sulfide as photo-reductant during daytime, at night the internal elemental sulfur of the cells gradually disappeared as the cells may have continued to grow, presumably using the internal S⁰ and O₂ for aerobic chemo-litho-trophy [28].

Below ~40 °C, in many of the mats in pools, rather than streams, a species of sulfide-oxidizing, filamentous, non-photosynthetic bacterium occurred. The gliding trichomes of Beggiatoa cf. leptomitiformis, migrated upward within the mat at nighttime (Figure 17), Nelson and Castenholz [19]. Although this strain of B. leptomitiformis oxidized sulfide and deposited S⁰ “internally”, the benefit was apparently, that of sulfide detoxification [29], since this species is, in fact, a chemoheterotroph, in which acetate is a favored substrate [21]. Ostracods were absent in these pools, probably because of inhibition by the higher sulfide, therefore, allowing a complete G. terebriformis top cover of the mat in daytime, mixed with Oscillatoria princeps [30] and Figure 17a, but at night is partially covered by the upward moving B. leptomitiformis population (Figure 17b). It was shown that B. leptomitiformis motility is negatively affected by high light and that its lower position in the mat in daytime is a result of a negative step-up photo-phobic response [19]. At night there was an aerotactic movement toward the O₂ at the surface of the mat [29], Figure 17b. The G. terebriformis/O. princeps population formed a cover in moderate light intensity in daytime. At night, in this scenario, the cyanobacteria remained in position, possibly because of the sulfide inhibition of gliding motility [18,31,32].

Figure 15. Portion of a southern Hunter’s stream at about 50 °C with high sulfide production, at night, showing Thermochromatium cf. tepidum (pink) at surface of soft substrate mixed with some dark areas of G. terebriformis.

Figure 15. Portion of a southern Hunter’s stream at about 50 °C with high sulfide production, at night, showing Thermochromatium cf. tepidum (pink) at surface of soft substrate mixed with some dark areas of G. terebriformis.

Figure 16. Swarm of swimming, ascending Thermochromatium tepidum (pink cloud at right) that had been covered by an opaque can for 2 h during 10 am on a cloudy day. In uncovered area, daytime cover of Geitlerinema terebriformis remained.

Figure 16. Swarm of swimming, ascending Thermochromatium tepidum (pink cloud at right) that had been covered by an opaque can for 2 h during 10 am on a cloudy day. In uncovered area, daytime cover of Geitlerinema terebriformis remained.

Figure 17. (a) Hunter’s pool at 9:25 am in April (~40 °C) with Geitlerinema terebriformis/Oscillatoria princeps cover (dark brown) but showing small patches of Beggiatoa leptomitiformis below the surface cover. Ostracods absent (Figure 2B in [18]); (b) Hunter’s pool at ~40 °C at 7:00 am (before sunrise, same day in April. Beggiatoa leptomitiformis (white) has migrated to the surface (complete darkness), with O₂-sulfide interface 0.2–0.3 mm below surface of mat, and covering the Geitlerinema terebriformis below (Figure 2C in [18]).

Figure 17. (a) Hunter’s pool at 9:25 am in April (~40 °C) with Geitlerinema terebriformis/Oscillatoria princeps cover (dark brown) but showing small patches of Beggiatoa leptomitiformis below the surface cover. Ostracods absent (Figure 2B in [18]); (b) Hunter’s pool at ~40 °C at 7:00 am (before sunrise, same day in April. Beggiatoa leptomitiformis (white) has migrated to the surface (complete darkness), with O₂-sulfide interface 0.2–0.3 mm below surface of mat, and covering the Geitlerinema terebriformis below (Figure 2C in [18]).

6. Discussion

The positioning of all phototrophic microorganisms and the herbivorous ostracod in the thermal gradient of Hunter’s Hot Springs is shown in a composite cartoon (Figure 18). It has been shown that in some cases the upper limit of some species is determined by temperature (e.g., Synechococcus high temperature type, Geitlerinema, ostracods, Thermochromatium, and probably Beggiatoa. But the lower limit of the massive distribution of Synechococcus is limited by Geitlerinema, and that of Geitlerinema and Synechococcus/Chloroflexus by the ostracods. Although it is known that Pleurocapsa and Calothrix are capable of growth at higher than the field distributions [26] the impact of ostracod grazing determines the actual distribution. All of the organisms present in Hunter’s Hot Springs tapered out because of suboptimal temperatures at ambient or <30 °C, and other phototrophs were only occasionally present (e.g., phycoerythrin-containing Nostoc, and filamentous green algae).

Although light intensity and daylength change from summer to winter (~15:09 summer; ~9:15 winter), the temperatures of the sources of Hunter’s Hot Springs remained the same, i.e., they were not influenced by the seasons. However, the zones of the phototrophs also remained more or less intact, most were retracted toward the water sources. This was particularly true for the Synechococcus and Geitlerinema zones that were able to recolonize or move upstream. However, the more stable gelatinous Chloroflexus mat was to some extent left behind at less than optimal temperature. The most conspicuous change was that the Pleurocapsa/Calothrix crust was left behind at a suboptimal temperature. The recolonization rate of this community was very slow. Therefore, it should be assumed that the photosynthetic and N₂-fixation rates of these cyanobacteria were at a suboptimal temperature, although this was not determined. The ostracods were present in similar densities in winter, and, as usual, were limiting the Geitlerinema mat at about 47 °C. It is possible that the effect of grazing on the crust of Pleurocapsa/Calothrix was more effective in winter because of the assumed lower productivity of these cyanobacteria, but this too was never established.

Figure 18. Cartoon summary of phototroph and ostracod distributions in Hunter’s streams. Upper portion with visible field distributions. Lower line below indicates Chloroflexus undermat distribution (47–68 °C) and ostracod, Pleurocapsa, Calothrix distributiions below 47 °C. The lower horizontal bars indicate ranges in culture of 4 Synechococcus thermotypes [3] and those of Chloroflexus, Oscillatoria (=Geitlerinema), Pleurocapsa, Calothrix, and Potamocypris (former name of the ostracod, Thermopsis thermophile). The range between arrows indicates the approximate optimal range for growth in culture.

Figure 18. Cartoon summary of phototroph and ostracod distributions in Hunter’s streams. Upper portion with visible field distributions. Lower line below indicates Chloroflexus undermat distribution (47–68 °C) and ostracod, Pleurocapsa, Calothrix distributiions below 47 °C. The lower horizontal bars indicate ranges in culture of 4 Synechococcus thermotypes [3] and those of Chloroflexus, Oscillatoria (=Geitlerinema), Pleurocapsa, Calothrix, and Potamocypris (former name of the ostracod, Thermopsis thermophile). The range between arrows indicates the approximate optimal range for growth in culture.