Dysfunction of Mitochondrial Dynamics in Drosophila Model of Diabetic Nephropathy
Abstract
:1. Introduction
2. Materials and Methods
2.1. Drosophila Stocks
2.2. Lifespan Assay
2.3. Immunohistochemistry
2.4. Western Blot Analysis
3. Results and discussion
3.1. Flies Fed Chronic High-Sucrose Diet Displayed Phenotypes of Previous Drosophila Models of Diabetes
3.2. Chronic High-Sucrose Diet Induced Mitochondrial Dynamic Imbalance
4. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
- Fineberg, D.; Jandeleit-Dahm, K.A.; Cooper, M.E. Diabetic nephropathy: Diagnosis and treatment. Nat. Rev. Endocrinol. 2013, 9, 713–723. [Google Scholar] [CrossRef] [PubMed]
- Forbes, J.M.; Thorburn, D.R. Mitochondrial dysfunction in diabetic kidney disease. Nat. Rev. Nephrol. 2018, 14, 291–312. [Google Scholar] [CrossRef] [PubMed]
- Imasawa, T.; Obre, E.; Bellance, N.; Lavie, J.; Imasawa, T.; Rigothier, C.; Delmas, Y.; Combe, C.; Lacombe, D.; Benard, G.; et al. High glucose repatterns human podocyte energy metabolism during differentiation and diabetic nephropathy. FASEB J. 2017, 31, 294–307. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Na, J.; Sweetwyne, M.T.; Park, A.S.; Susztak, K.; Cagan, R.L. Diet-Induced Podocyte Dysfunction in Drosophila and Mammals. Cell Rep. 2015, 12, 636–647. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Musselman, L.P.; Fink, J.L.; Narzinski, K.; Ramachandran, P.V.; Hathiramani, S.S.; Cagan, R.L.; Baranski, T.J. A high-sugar diet produces obesity and insulin resistance in wild-type Drosophila. Dis. Model. Mech. 2011, 4, 842–849. [Google Scholar] [CrossRef] [Green Version]
- Ecker, A.; Gonzaga, T.; Seeger, R.L.; Santos, M.M.D.; Loreto, J.S.; Boligon, A.A.; Meinerz, D.F.; Lugokenski, T.H.; Rocha, J.; Barbosa, N.V. High-sucrose diet induces diabetic-like phenotypes and oxidative stress in Drosophila melanogaster: Protective role of Syzygium cumini and Bauhinia forficata. Biomed. Pharmacother. 2017, 89, 605–616. [Google Scholar] [CrossRef]
- Bhatia, D.; Capili, A.; Choi, M.E. Mitochondrial dysfunction in kidney injury, inflammation, and disease: Potential therapeutic approaches. Kidney Res. Clin. Pract. 2020, 39, 244–258. [Google Scholar] [CrossRef]
- Kuo, C.W.; Shen, C.J.; Tung, Y.T.; Chen, H.L.; Chen, Y.H.; Chang, W.H.; Cheng, K.C.; Yang, S.H.; Chen, C.M. Extracellular superoxide dismutase ameliorates streptozotocin-induced rat diabetic nephropathy via inhibiting the ROS/ERK1/2 signaling. Life Sci. 2015, 135, 77–86. [Google Scholar] [CrossRef]
- Doublier, S.; Salvidio, G.; Lupia, E.; Ruotsalainen, V.; Verzola, D.; Deferrari, G.; Camussi, G. Nephrin expression is reduced in human diabetic nephropathy: Evidence for a distinct role for glycated albumin and angiotensin II. Diabetes 2003, 52, 1023–1030. [Google Scholar] [CrossRef] [Green Version]
- Reidy, K.; Kang, H.M.; Hostetter, T.; Susztak, K. Molecular mechanisms of diabetic kidney disease. J. Clin. Investig. 2014, 124, 2333–2340. [Google Scholar] [CrossRef]
- Friedman, J.R.; Nunnari, J. Mitochondrial form and function. Nature 2014, 505, 335–343. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Qi, H.; Casalena, G.; Shi, S.; Yu, L.; Ebefors, K.; Sun, Y.; Zhang, W.; D’Agati, V.; Schlondorff, D.; Haraldsson, B.; et al. Glomerular Endothelial Mitochondrial Dysfunction Is Essential and Characteristic of Diabetic Kidney Disease Susceptibility. Diabetes 2017, 66, 763–778. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Qi, W.; Keenan, H.A.; Li, Q.; Ishikado, A.; Kannt, A.; Sadowski, T.; Yorek, M.A.; Wu, I.H.; Lockhart, S.; Coppey, L.J.; et al. Pyruvate kinase M2 activation may protect against the progression of diabetic glomerular pathology and mitochondrial dysfunction. Nat. Med. 2017, 23, 753–762. [Google Scholar] [CrossRef] [PubMed]
- Coughlan, M.T.; Sharma, K. Challenging the dogma of mitochondrial reactive oxygen species overproduction in diabetic kidney disease. Kidney Int. 2016, 90, 272–279. [Google Scholar] [CrossRef] [PubMed]
- Nishikawa, T.; Brownlee, M.; Araki, E. Mitochondrial reactive oxygen species in the pathogenesis of early diabetic nephropathy. J. Diabetes Investig. 2015, 6, 137–139. [Google Scholar] [CrossRef]
- Kaneda, K.; Iwao, J.; Sakata, N.; Takebayashi, S. Correlation between mitochondrial enlargement in renal proximal tubules and microalbuminuria in rats with early streptozotocin-induced diabetes. Acta Pathol. Jpn. 1992, 42, 855–860. [Google Scholar] [CrossRef]
- Takebayashi, S.; Kaneda, K. Mitochondrial derangement: Possible initiator of microalbuminuria in NIDDM. J. Diabet. Complicat. 1991, 5, 104–106. [Google Scholar] [CrossRef]
- Weavers, H.; Prieto-Sanchez, S.; Grawe, F.; Garcia-Lopez, A.; Artero, R.; Wilsch-Brauninger, M.; Ruiz-Gomez, M.; Skaer, H.; Denholm, B. The insect nephrocyte is a podocyte-like cell with a filtration slit diaphragm. Nature 2009, 457, 322–326. [Google Scholar] [CrossRef] [Green Version]
- Zhuang, S.; Shao, H.; Guo, F.; Trimble, R.; Pearce, E.; Abmayr, S.M. Sns and Kirre, the Drosophila orthologs of Nephrin and Neph1, direct adhesion, fusion and formation of a slit diaphragm-like structure in insect nephrocytes. Development 2009, 136, 2335–2344. [Google Scholar] [CrossRef] [Green Version]
- Navrotskaya, V.; Oxenkrug, G.; Vorobyova, L.; Summergrad, P. Attenuation of high sucrose diet-induced insulin resistance in tryptophan 2,3-dioxygenase deficient Drosophila melanogaster vermilion mutants. Integr. Obes. Diabetes 2015, 1, 93–95. [Google Scholar] [CrossRef] [Green Version]
- Morris, S.N.; Coogan, C.; Chamseddin, K.; Fernandez-Kim, S.O.; Kolli, S.; Keller, J.N.; Bauer, J.H. Development of diet-induced insulin resistance in adult Drosophila melanogaster. Biochim. Biophys. Acta 2012, 1822, 1230–1237. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Pasco, M.Y.; Leopold, P. High sugar-induced insulin resistance in Drosophila relies on the lipocalin Neural Lazarillo. PLoS ONE 2012, 7, e36583. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- DiAngelo, J.R.; Birnbaum, M.J. Regulation of fat cell mass by insulin in Drosophila melanogaster. Mol. Cell Biol. 2009, 29, 6341–6352. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Giacomello, M.; Pyakurel, A.; Glytsou, C.; Scorrano, L. The cell biology of mitochondrial membrane dynamics. Nat. Rev. Mol. Cell Biol 2020, 21, 204–224. [Google Scholar] [CrossRef]
- Tilokani, L.; Nagashima, S.; Paupe, V.; Prudent, J. Mitochondrial dynamics: Overview of molecular mechanisms. Essays Biochem. 2018, 62, 341–360. [Google Scholar] [CrossRef] [Green Version]
- Detmer, S.A.; Chan, D.C. Functions and dysfunctions of mitochondrial dynamics. Nat. Rev. Mol. Cell Biol. 2007, 8, 870–879. [Google Scholar] [CrossRef]
- Ni, H.M.; Williams, J.A.; Ding, W.X. Mitochondrial dynamics and mitochondrial quality control. Redox Biol. 2015, 4, 6–13. [Google Scholar] [CrossRef] [Green Version]
- Chan, D.C. Mitochondrial Dynamics and Its Involvement in Disease. Annu. Rev. Pathol. 2020, 15, 235–259. [Google Scholar] [CrossRef] [Green Version]
- Galvan, D.L.; Green, N.H.; Danesh, F.R. The hallmarks of mitochondrial dysfunction in chronic kidney disease. Kidney Int. 2017, 92, 1051–1057. [Google Scholar] [CrossRef]
- Che, R.; Yuan, Y.; Huang, S.; Zhang, A. Mitochondrial dysfunction in the pathophysiology of renal diseases. Am. J. Physiol. Renal. Physiol. 2014, 306, F367–F378. [Google Scholar] [CrossRef]
- Sas, K.M.; Kayampilly, P.; Byun, J.; Nair, V.; Hinder, L.M.; Hur, J.; Zhang, H.; Lin, C.; Qi, N.R.; Michailidis, G.; et al. Tissue-specific metabolic reprogramming drives nutrient flux in diabetic complications. JCI Insight 2016, 1, e86976. [Google Scholar] [CrossRef] [PubMed]
- Sabouny, R.; Shutt, T.E. Reciprocal Regulation of Mitochondrial Fission and Fusion. Trends Biochem. Sci. 2020, 45, 564–577. [Google Scholar] [CrossRef] [PubMed]
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Kim, K.; Cha, S.J.; Choi, H.-J.; Kang, J.S.; Lee, E.Y. Dysfunction of Mitochondrial Dynamics in Drosophila Model of Diabetic Nephropathy. Life 2021, 11, 67. https://doi.org/10.3390/life11010067
Kim K, Cha SJ, Choi H-J, Kang JS, Lee EY. Dysfunction of Mitochondrial Dynamics in Drosophila Model of Diabetic Nephropathy. Life. 2021; 11(1):67. https://doi.org/10.3390/life11010067
Chicago/Turabian StyleKim, Kiyoung, Sun Joo Cha, Hyun-Jun Choi, Jeong Suk Kang, and Eun Young Lee. 2021. "Dysfunction of Mitochondrial Dynamics in Drosophila Model of Diabetic Nephropathy" Life 11, no. 1: 67. https://doi.org/10.3390/life11010067