Antibodies 2012, 1(3), 273-283; doi:10.3390/antib1030273
Immunochemical Analysis of the Antimalarial Drugs Artemisinin and Artesunate
1
Department of Pharmacognosy, Faculty of Pharmaceutical Sciences, Kyushu University, Fukuoka 812-8582, Japan
2
Faculty of Pharmaceutical Sciences, Khon Kaen University, Khon Kaen, 40002, Thailand
3
Faculty of Pharmaceutical Sciences, Ubon Ratchathani University, Ubon Ratchathani, 34190, Thailand
4
Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok, Thailand
5
Faculty of Pharmacy, Rangsit University, Pathumthani, Thailand
6
Faculty of Pharmaceutical Sciences, Nagasaki International University, Huis Ten Bosch 2825-7, Sasebo, Nagasaki 859-3298, Japan
*
Author to whom correspondence should be addressed.
Received: 3 September 2012 / Revised: 19 October 2012 / Accepted: 29 October 2012 / Published: 2 November 2012
(This article belongs to the Special Issue Monoclonal Antibody Preparation against Natural Product and Its Application)
Abstract
We prepared a monoclonal antibody (mAb 1C1) showing specificity for artemisinin (AM) and artesunate (AS), and we developed an indirect competitive enzyme-linked immunosorbent assay (icELISA) using this novel mAb. Moreover, we prepared a recombinant antibody derived from mAb 1C1 in order to overcome insufficient mAb production by hybridoma culture. A recombinant antigen-binding fragment (Fab) was easily constructed using antibody manipulation technologies and was produced by microorganisms in high yield. We herein review immunochemical approaches for analysis of the antimalarial drugs AM and AS that were able to yield analysis results for multiple samples in a short period of time using simple and reliable protocols.Keywords:
artemisinin; artesunate; Artemisia annua; monoclonal antibody; recombinant Fab; ELISA
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).
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MDPI and ACS Style
Tanaka, H.; Paudel, M.K.; Takei, A.; Sakoda, J.; Juengwatanatrakul, T.; Sasaki-Tabata, K.; Putalun, W.; De-Eknamkul, W.; Matangkasombut, O.; Shoyama, Y.; Morimoto, S. Immunochemical Analysis of the Antimalarial Drugs Artemisinin and Artesunate. Antibodies 2012, 1, 273-283.
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