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Immunochemical Analysis of the Antimalarial Drugs Artemisinin and Artesunate
Department of Pharmacognosy, Faculty of Pharmaceutical Sciences, Kyushu University, Fukuoka 812-8582, Japan
Faculty of Pharmaceutical Sciences, Khon Kaen University, Khon Kaen, 40002, Thailand
Faculty of Pharmaceutical Sciences, Ubon Ratchathani University, Ubon Ratchathani, 34190, Thailand
Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok, Thailand
Faculty of Pharmacy, Rangsit University, Pathumthani, Thailand
Faculty of Pharmaceutical Sciences, Nagasaki International University, Huis Ten Bosch 2825-7, Sasebo, Nagasaki 859-3298, Japan
* Author to whom correspondence should be addressed.
Received: 3 September 2012; in revised form: 19 October 2012 / Accepted: 29 October 2012 / Published: 2 November 2012
Abstract: We prepared a monoclonal antibody (mAb 1C1) showing specificity for artemisinin (AM) and artesunate (AS), and we developed an indirect competitive enzyme-linked immunosorbent assay (icELISA) using this novel mAb. Moreover, we prepared a recombinant antibody derived from mAb 1C1 in order to overcome insufficient mAb production by hybridoma culture. A recombinant antigen-binding fragment (Fab) was easily constructed using antibody manipulation technologies and was produced by microorganisms in high yield. We herein review immunochemical approaches for analysis of the antimalarial drugs AM and AS that were able to yield analysis results for multiple samples in a short period of time using simple and reliable protocols.
Keywords: artemisinin; artesunate; Artemisia annua; monoclonal antibody; recombinant Fab; ELISA
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Tanaka, H.; Paudel, M.K.; Takei, A.; Sakoda, J.; Juengwatanatrakul, T.; Sasaki-Tabata, K.; Putalun, W.; De-Eknamkul, W.; Matangkasombut, O.; Shoyama, Y.; Morimoto, S. Immunochemical Analysis of the Antimalarial Drugs Artemisinin and Artesunate. Antibodies 2012, 1, 273-283.
Tanaka H, Paudel MK, Takei A, Sakoda J, Juengwatanatrakul T, Sasaki-Tabata K, Putalun W, De-Eknamkul W, Matangkasombut O, Shoyama Y, Morimoto S. Immunochemical Analysis of the Antimalarial Drugs Artemisinin and Artesunate. Antibodies. 2012; 1(3):273-283.
Tanaka, Hiroyuki; Paudel, Madan K.; Takei, Ayako; Sakoda, Junichi; Juengwatanatrakul, Thaweesak; Sasaki-Tabata, Kaori; Putalun, Waraporn; De-Eknamkul, Wanchai; Matangkasombut, Oraphan; Shoyama, Yukihiro; Morimoto, Satoshi. 2012. "Immunochemical Analysis of the Antimalarial Drugs Artemisinin and Artesunate." Antibodies 1, no. 3: 273-283.