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Antibodies 2012, 1(3), 273-283; doi:10.3390/antib1030273
Review

Immunochemical Analysis of the Antimalarial Drugs Artemisinin and Artesunate

1,* , 1, 1, 1, 3, 1, 2, 4, 5, 6 and 1
Received: 3 September 2012; in revised form: 19 October 2012 / Accepted: 29 October 2012 / Published: 2 November 2012
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Abstract: We prepared a monoclonal antibody (mAb 1C1) showing specificity for artemisinin (AM) and artesunate (AS), and we developed an indirect competitive enzyme-linked immunosorbent assay (icELISA) using this novel mAb. Moreover, we prepared a recombinant antibody derived from mAb 1C1 in order to overcome insufficient mAb production by hybridoma culture. A recombinant antigen-binding fragment (Fab) was easily constructed using antibody manipulation technologies and was produced by microorganisms in high yield. We herein review immunochemical approaches for analysis of the antimalarial drugs AM and AS that were able to yield analysis results for multiple samples in a short period of time using simple and reliable protocols.
Keywords: artemisinin; artesunate; Artemisia annua; monoclonal antibody; recombinant Fab; ELISA artemisinin; artesunate; Artemisia annua; monoclonal antibody; recombinant Fab; ELISA
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Tanaka, H.; Paudel, M.K.; Takei, A.; Sakoda, J.; Juengwatanatrakul, T.; Sasaki-Tabata, K.; Putalun, W.; De-Eknamkul, W.; Matangkasombut, O.; Shoyama, Y.; Morimoto, S. Immunochemical Analysis of the Antimalarial Drugs Artemisinin and Artesunate. Antibodies 2012, 1, 273-283.

AMA Style

Tanaka H, Paudel MK, Takei A, Sakoda J, Juengwatanatrakul T, Sasaki-Tabata K, Putalun W, De-Eknamkul W, Matangkasombut O, Shoyama Y, Morimoto S. Immunochemical Analysis of the Antimalarial Drugs Artemisinin and Artesunate. Antibodies. 2012; 1(3):273-283.

Chicago/Turabian Style

Tanaka, Hiroyuki; Paudel, Madan K.; Takei, Ayako; Sakoda, Junichi; Juengwatanatrakul, Thaweesak; Sasaki-Tabata, Kaori; Putalun, Waraporn; De-Eknamkul, Wanchai; Matangkasombut, Oraphan; Shoyama, Yukihiro; Morimoto, Satoshi. 2012. "Immunochemical Analysis of the Antimalarial Drugs Artemisinin and Artesunate." Antibodies 1, no. 3: 273-283.


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