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Antibodies 2012, 1(3), 259-272; doi:10.3390/antib1030259
Article

Quality Control System for Beer Developed with Monoclonal Antibodies Specific to Barley Lipid Transfer Protein

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1 Kyoto College of Nutritional & Medical, Setogawa-cho 18, Saga Tenryuji, Ukyo-ku, Kyoto 616-8376, Japan 2 Department of Food and Nutrition, School of Human Cultures, The University of Shiga Prefecture, Hassaka-cho 2500, Hikone, Shiga 522-8533, Japan 3 Department of Life and Living, Osaka Seikei College, Aikawa 3-10-62, Higashiyodogawa, Osaka 533-0007, Japan 4 Department of Food and Nutrition, Kyoto Women’s University, Kitahiyoshi-cho 35, Imakumano, Higashiyama-ku, Kyoto 605-8501, Japan 5 Suntory Business Expert Limited, Wakayamadai, Shimamoto-cho, Mishima-gun, Osaka 618-8503, Japan
* Author to whom correspondence should be addressed.
Received: 17 September 2012 / Revised: 28 September 2012 / Accepted: 9 October 2012 / Published: 16 October 2012
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Abstract

Non-specific lipid transfer protein (LTP) in barley grain reacted with the IgE in sera drawn from food allergy patients. A sandwich-type of enzyme-linked immunosorbent assay (ELISA) was developed with mouse monoclonal antibodies raised against LTP purified with barley flour. This ELISA showed a practical working range of 0.3–3 ng/mL and no cross-reactivity with wheat, adlay and rye. Using this ELISA, LTP was determined in several types of barley-foods, including fermented foods such as malt vinegar, barley-malt miso and beer. LTP content in beer of the same kind was approximately constant, even if manufacturing factory and production days were different. Not only as a factor of foam formation and stability but also as an allergen, controlling and monitoring of LTP in beer should be considered. Taken together, our LTP-detecting ELISA can be proposed as an appropriate system for the quality control of beer.
Keywords: lipid transfer protein; beer; ELISA; food allergy; monoclonal antibody lipid transfer protein; beer; ELISA; food allergy; monoclonal antibody
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).
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Murakami-Yamaguchi, Y.; Hirose, J.; Kizu, K.; Okazaki, F.; Fujii, W.; Narita, H. Quality Control System for Beer Developed with Monoclonal Antibodies Specific to Barley Lipid Transfer Protein. Antibodies 2012, 1, 259-272.

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