Antibodies 2012, 1(3), 259-272; doi:10.3390/antib1030259
Article

Quality Control System for Beer Developed with Monoclonal Antibodies Specific to Barley Lipid Transfer Protein

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Received: 17 September 2012; in revised form: 28 September 2012 / Accepted: 9 October 2012 / Published: 16 October 2012
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: Non-specific lipid transfer protein (LTP) in barley grain reacted with the IgE in sera drawn from food allergy patients. A sandwich-type of enzyme-linked immunosorbent assay (ELISA) was developed with mouse monoclonal antibodies raised against LTP purified with barley flour. This ELISA showed a practical working range of 0.3–3 ng/mL and no cross-reactivity with wheat, adlay and rye. Using this ELISA, LTP was determined in several types of barley-foods, including fermented foods such as malt vinegar, barley-malt miso and beer. LTP content in beer of the same kind was approximately constant, even if manufacturing factory and production days were different. Not only as a factor of foam formation and stability but also as an allergen, controlling and monitoring of LTP in beer should be considered. Taken together, our LTP-detecting ELISA can be proposed as an appropriate system for the quality control of beer.
Keywords: lipid transfer protein; beer; ELISA; food allergy; monoclonal antibody
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MDPI and ACS Style

Murakami-Yamaguchi, Y.; Hirose, J.; Kizu, K.; Okazaki, F.; Fujii, W.; Narita, H. Quality Control System for Beer Developed with Monoclonal Antibodies Specific to Barley Lipid Transfer Protein. Antibodies 2012, 1, 259-272.

AMA Style

Murakami-Yamaguchi Y, Hirose J, Kizu K, Okazaki F, Fujii W, Narita H. Quality Control System for Beer Developed with Monoclonal Antibodies Specific to Barley Lipid Transfer Protein. Antibodies. 2012; 1(3):259-272.

Chicago/Turabian Style

Murakami-Yamaguchi, Yukie; Hirose, Junko; Kizu, Kumiko; Okazaki, Fumiko; Fujii, Wataru; Narita, Hiroshi. 2012. "Quality Control System for Beer Developed with Monoclonal Antibodies Specific to Barley Lipid Transfer Protein." Antibodies 1, no. 3: 259-272.

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