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Quality Control System for Beer Developed with Monoclonal Antibodies Specific to Barley Lipid Transfer Protein
Kyoto College of Nutritional & Medical, Setogawa-cho 18, Saga Tenryuji, Ukyo-ku, Kyoto 616-8376, Japan
Department of Food and Nutrition, School of Human Cultures, The University of Shiga Prefecture, Hassaka-cho 2500, Hikone, Shiga 522-8533, Japan
Department of Life and Living, Osaka Seikei College, Aikawa 3-10-62, Higashiyodogawa, Osaka 533-0007, Japan
Department of Food and Nutrition, Kyoto Women’s University, Kitahiyoshi-cho 35, Imakumano, Higashiyama-ku, Kyoto 605-8501, Japan
Suntory Business Expert Limited, Wakayamadai, Shimamoto-cho, Mishima-gun, Osaka 618-8503, Japan
* Author to whom correspondence should be addressed.
Received: 17 September 2012; in revised form: 28 September 2012 / Accepted: 9 October 2012 / Published: 16 October 2012
Abstract: Non-specific lipid transfer protein (LTP) in barley grain reacted with the IgE in sera drawn from food allergy patients. A sandwich-type of enzyme-linked immunosorbent assay (ELISA) was developed with mouse monoclonal antibodies raised against LTP purified with barley flour. This ELISA showed a practical working range of 0.3–3 ng/mL and no cross-reactivity with wheat, adlay and rye. Using this ELISA, LTP was determined in several types of barley-foods, including fermented foods such as malt vinegar, barley-malt miso and beer. LTP content in beer of the same kind was approximately constant, even if manufacturing factory and production days were different. Not only as a factor of foam formation and stability but also as an allergen, controlling and monitoring of LTP in beer should be considered. Taken together, our LTP-detecting ELISA can be proposed as an appropriate system for the quality control of beer.
Keywords: lipid transfer protein; beer; ELISA; food allergy; monoclonal antibody
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MDPI and ACS Style
Murakami-Yamaguchi, Y.; Hirose, J.; Kizu, K.; Okazaki, F.; Fujii, W.; Narita, H. Quality Control System for Beer Developed with Monoclonal Antibodies Specific to Barley Lipid Transfer Protein. Antibodies 2012, 1, 259-272.
Murakami-Yamaguchi Y, Hirose J, Kizu K, Okazaki F, Fujii W, Narita H. Quality Control System for Beer Developed with Monoclonal Antibodies Specific to Barley Lipid Transfer Protein. Antibodies. 2012; 1(3):259-272.
Murakami-Yamaguchi, Yukie; Hirose, Junko; Kizu, Kumiko; Okazaki, Fumiko; Fujii, Wataru; Narita, Hiroshi. 2012. "Quality Control System for Beer Developed with Monoclonal Antibodies Specific to Barley Lipid Transfer Protein." Antibodies 1, no. 3: 259-272.