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Water 2018, 10(1), 82; https://doi.org/10.3390/w10010082

Bioassessment of a Drinking Water Reservoir Using Plankton: High Throughput Sequencing vs. Traditional Morphological Method

1,2,†
,
1,2,†
,
1,2,* , 2,3
,
1,2
,
1,2
,
2,4
and
2,4
1
Key Laboratory of Ecological Security for Water Source Region of Middle Route Project of South-North Water Diversion of Henan Province, College of Agricultural Engineering, Nanyang Normal University, Nanyang 473061, Henan, China
2
Collaborative Innovation Center of Water Security for Water Source Region of Middle Route Project of South-North Water Diversion of Henan Province, Nanyang Normal University, Nanyang 473061, Henan, China
3
Department of Environmental Science and Management, Portland State University, Portland, OR 97207, USA
4
Emergency Centre for Environmental Monitoring of the Canal Head of Middle Route Project of South-North Water Division, Xichuan 474475, Henan, China
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Received: 25 November 2017 / Revised: 12 January 2018 / Accepted: 15 January 2018 / Published: 18 January 2018
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Abstract

Drinking water safety is increasingly perceived as one of the top global environmental issues. Plankton has been commonly used as a bioindicator for water quality in lakes and reservoirs. Recently, DNA sequencing technology has been applied to bioassessment. In this study, we compared the effectiveness of the 16S and 18S rRNA high throughput sequencing method (HTS) and the traditional optical microscopy method (TOM) in the bioassessment of drinking water quality. Five stations reflecting different habitats and hydrological conditions in Danjiangkou Reservoir, one of the largest drinking water reservoirs in Asia, were sampled May 2016. Non-metric multi-dimensional scaling (NMDS) analysis showed that plankton assemblages varied among the stations and the spatial patterns revealed by the two methods were consistent. The correlation between TOM and HTS in a symmetric Procrustes analysis was 0.61, revealing overall good concordance between the two methods. Procrustes analysis also showed that site-specific differences between the two methods varied among the stations. Station Heijizui (H), a site heavily influenced by two tributaries, had the largest difference while station Qushou (Q), a confluence site close to the outlet dam, had the smallest difference between the two methods. Our results show that DNA sequencing has the potential to provide consistent identification of taxa, and reliable bioassessment in a long-term biomonitoring and assessment program for drinking water reservoirs. View Full-Text
Keywords: Danjiangkou Reservoir; plankton; high throughput sequencing; generalized procrustes analysis; bioassessment Danjiangkou Reservoir; plankton; high throughput sequencing; generalized procrustes analysis; bioassessment
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Gao, W.; Chen, Z.; Li, Y.; Pan, Y.; Zhu, J.; Guo, S.; Hu, L.; Huang, J. Bioassessment of a Drinking Water Reservoir Using Plankton: High Throughput Sequencing vs. Traditional Morphological Method. Water 2018, 10, 82.

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