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Genes 2018, 9(3), 156; https://doi.org/10.3390/genes9030156

Genome-Wide Analysis of the PYL Gene Family and Identification of PYL Genes That Respond to Abiotic Stress in Brassica napus

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College of Agronomy and Biotechnology, Chongqing Engineering Research Center for Rapeseed, Southwest University, Chongqing 400716, China
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Academy of Agricultural Sciences, Southwest University, Chongqing 400716, China
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Author to whom correspondence should be addressed.
Received: 15 January 2018 / Revised: 26 February 2018 / Accepted: 6 March 2018 / Published: 12 March 2018
(This article belongs to the Special Issue Estimating Phylogenies from Large Genomic Datasets)
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Abstract

Abscisic acid (ABA) is an endogenous phytohormone that plays important roles in the regulation of plant growth, development, and stress responses. The pyrabactin resistance 1-like (PYR/PYL) protein is a core regulatory component of ABA signaling networks in plants. However, no details regarding this family in Brassica napus are available. Here, 46 PYLs were identified in the B. napus genome. Based on phylogenetic analysis, BnPYR1 and BnPYL1-3 belong to subfamily I, BnPYL7-10 belong to subfamily II, and BnPYL4-6 and BnPYL11-13 belong to subfamily III. Analysis of BnPYL conserved motifs showed that every subfamily contained four common motifs. By predicting cis-elements in the promoters, we found that all BnPYL members contained hormone- and stress-related elements and that expression levels of most BnPYLs were relatively higher in seeds at the germination stage than those in other organs or at other developmental stages. Gene Ontology (GO) enrichment showed that BnPYL genes mainly participate in responses to stimuli. To identify crucial PYLs mediating the response to abiotic stress in B. napus, expression changes in 14 BnPYL genes were determined by quantitative real-time RT-PCR after drought, heat, and salinity treatments, and identified BnPYR1-3, BnPYL1-2, and BnPYL7-2 in respond to abiotic stresses. The findings of this study lay a foundation for further investigations of PYL genes in B. napus. View Full-Text
Keywords: Brassica napus; abscisic acid; PYL; gene expression pattern; abiotic stress Brassica napus; abscisic acid; PYL; gene expression pattern; abiotic stress
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Di, F.; Jian, H.; Wang, T.; Chen, X.; Ding, Y.; Du, H.; Lu, K.; Li, J.; Liu, L. Genome-Wide Analysis of the PYL Gene Family and Identification of PYL Genes That Respond to Abiotic Stress in Brassica napus. Genes 2018, 9, 156.

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