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Genes 2018, 9(2), 81; doi:10.3390/genes9020081

Pathogenicity Islands Distribution in Non-O157 Shiga Toxin-Producing Escherichia coli (STEC)

Immunochemistry and Biotechnology Laboratory, Faculty of Veterinary Sciences, Tandil Veterinary Research Center, CONICET-CIC-UNCPBA, University Campus, Tandil 7000, Buenos Aires, Argentina
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Received: 29 December 2017 / Revised: 6 February 2018 / Accepted: 7 February 2018 / Published: 10 February 2018
(This article belongs to the Special Issue Genetics and Genomics of Foodborne Pathogens)
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Abstract

Shiga toxin-producing Escherichia coli (STEC) are foodborne pathogens associated with outbreaks and hemolytic-uremic syndrome. Cattle and meat foods are the main reservoir and infection source, respectively. Pathogenicity islands (PAIs) play an important role in STEC pathogenicity, and non-locus of the enterocyte effacement(LEE) effector (nle) genes present on them encode translocated substrates of the type III secretion system. A molecular risk assessment based on the evaluation of the nle content has been used to predict which STEC strains pose a risk to humans. The goal was to investigate the distribution of the PAIs OI (O-island)-36 (nleB2, nleC, nleH1-1, nleD), OI-57 (nleG2-3, nleG5-2, nleG6-2), OI-71 (nleA, nleF, nleG, nleG2-1, nleG9, nleH1-2) and OI-122 (ent/espL2, nleB, nleE, Z4321, Z4326, Z4332, Z4333) among 204 clinical, food and animal isolates belonging to 52 non-O157:H7 serotypes. Differences in the frequencies of genetic markers and a wide spectrum of PAI virulence profiles were found. In most LEE-negative strains, only module 1 (Z4321) of OI-122 was present. However, some unusual eae-negative strains were detected, which carried other PAI genes. The cluster analysis, excluding isolates that presented no genes, defined two major groups: eae-negative (determined as seropathotypes (SPTs) D, E or without determination, isolated from cattle or food) and eae-positive (mostly identified as SPTs B, C, or not determined). View Full-Text
Keywords: STEC; Pathogenicity islands; nle genes; seropathotypes STEC; Pathogenicity islands; nle genes; seropathotypes
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MDPI and ACS Style

Soledad-Cadona, J.; Bustamante, A.V.; González, J.; Mariel-Sanso, A. Pathogenicity Islands Distribution in Non-O157 Shiga Toxin-Producing Escherichia coli (STEC). Genes 2018, 9, 81.

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