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Genes 2018, 9(2), 28; https://doi.org/10.3390/genes9020028

Proteomic Analysis of Methanonatronarchaeum thermophilum AMET1, a Representative of a Putative New Class of Euryarchaeota, “Methanonatronarchaeia”

1
Institute of Catalysis, CSIC, 28049 Madrid, Spain
2
Winogradsky Institute of Microbiology, Research Centre for Biotechnology, Russian Academy of Sciences, Prospect 60-let Octyabrya 7/2, 117312 Moscow, Russia
3
Department of Biotechnology, Delft University of Technology, van der Maasweg 9, 2629 HZ Delft, The Netherlands
4
National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD 20894, USA
5
Proteomics Facility, Centro Nacional de Biotecnología, CSIC, 28049 Madrid, Spain
Current address: School of Chemistry, Bangor University, Bangor LL57 2UW, UK.
*
Author to whom correspondence should be addressed.
Received: 18 December 2017 / Revised: 9 January 2018 / Accepted: 10 January 2018 / Published: 23 January 2018
(This article belongs to the Special Issue Genetics and Genomics of Extremophiles)
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Abstract

The recently discovered Methanonatronarchaeia are extremely halophilic and moderately thermophilic methyl-reducing methanogens representing a novel class-level lineage in the phylum Euryarchaeota related to the class Halobacteria. Here we present a detailed analysis of 1D-nano liquid chromatography–electrospray ionization tandem mass spectrometry data obtained for “Methanonatronarchaeum thermophilum” AMET1 grown in different physiological conditions, including variation of the growth temperature and substrates. Analysis of these data allows us to refine the current understanding of the key biosynthetic pathways of this triple extremophilic methanogenic euryarchaeon and identify proteins that are likely to be involved in its response to growth condition changes. View Full-Text
Keywords: proteomics; methanogenesis; halophiles; Methanonatronarchaeia; genomics proteomics; methanogenesis; halophiles; Methanonatronarchaeia; genomics
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Ferrer, M.; Sorokin, D.Y.; Wolf, Y.I.; Ciordia, S.; Mena, M.C.; Bargiela, R.; Koonin, E.V.; Makarova, K.S. Proteomic Analysis of Methanonatronarchaeum thermophilum AMET1, a Representative of a Putative New Class of Euryarchaeota, “Methanonatronarchaeia”. Genes 2018, 9, 28.

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